Citation: Xinyu ZENG, Guhua TANG, Jianming OUYANG. Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals[J]. Chinese Journal of Inorganic Chemistry, ;2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374 shu

Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals

College of Chemistry and Materials Science, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: Jianming OUYANG, toyjm@jnu.edu.cn
Received Date: 10 October 2023
Revised Date: 20 May 2024

Figures(11)

To explore the regulatory effect of Desmodium styracifolium polysaccharides before and after carboxymethylation (DSPs) on the nucleation, growth and aggregation of calcium oxalate (CaC₂O₄) crystals, the CaC₂O₄ crystals regulated by DSPs with different carboxyl (—COOH) contents (mass fractions) were characterized by FTIR, powder X-ray diffraction, scanning electron microscope, ζ potential and thermogravimetric analysis (TGA). The protective effect of DSPs on human renal proximal tubular epithelial cells (HK-2 cells) was evaluated by examining adhesion molecule expression and nano-COM (nano calcium oxalate monohydrate) cell adhesion. The results show that DSPs can inhibit the growth of COM crystals, induce the formation of calcium oxalate dihydrate (COD) crystals, increase the absolute value of ζ potential on the surface of CaC₂O₄ crystals, and inhibit aggregation between crystals. When the —COOH content in the polysaccharide increased from 1.17% to 7.45%, 12.2% and 17.7%, the ability of DSP0, DSP1, DSP2 and DSP3 to regulate the growth of CaC₂O₄ crystals increased in sequence. TGA shows that polysaccharides are adsorbed in the crystal. In the presence of 0.2 g·L^-1 DSPs, the mass fractions of DSP0, DSP1, DSP2 and DSP3 incorporated into the crystals were 1.54%, 2.94%, 7.96% and 8.12%, respectively. Cell experiments show that DSPs can significantly reduce the expression of adhesion molecules CD44 and Annexin A2 on the surface of HK-2 cells, and reduce the amount of nano-COM adhesion on the cell surface. DSPs can inhibit the nucleation, aggregation, and growth of CaC₂O₄ crystals, and reduce the adhesion of nano-COM on renal epithelial cells, all these are beneficial to inhibit the formation of CaC₂O₄ kidney stones, among which DSP3 with the highest degree of carboxylation has the best effect.
- calcium oxalate,
- crystallization regulation,
- crystal adhesion molecule,
- scanning electron microscope,
- kidney stone
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