Citation: Cun WANG, Xiao-Chuan ZOU, Yuan-Bo HUANG, Jing HU, Ming-Chuan DENG, Xia FENG. Electrochemical aptamer sensor for thrombin based on cerium complex signal probe[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(10): 1931-1940. doi: 10.11862/CJIC.2023.162 shu

Electrochemical aptamer sensor for thrombin based on cerium complex signal probe

College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China

Corresponding author: Xiao-Chuan ZOU, zxcvip2003@163.com
Received Date: 3 January 2023
Revised Date: 17 September 2023

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In this work, a series of cerium coordination polymers (Ce-COPs) with different morphology and electro- chemical signals were synthesized by a simple hydrothermal method using Ce³⁺ as the central ion and N, N-dimethyl- formamide as the organic ligand through temperature regulation. The polyhedral Ce-COP with the largest electro- chemical signal was selected as the signal probe. Then, a sensitive thrombin (TB) aptamer sensor was designed through the specific recognition between TB and TB aptamer. Under the optimal experimental conditions, the linear range for TB detection was from 1.0 fmol·L^-1 to 1.0 nmol·L^-1, and the detection limit was 0.94 fmol·L^-1. In addition, our method is similar to the results of commercial human thrombin (TM) ELISA reagent detection. In a word, our biosensor possesses good sensitivity, specificity, selectivity, and stability.
- cerium coordination polymer,
- sensor,
- thrombin,
- aptamer,
- electrochemistry
1. [1]
  Gómez-Arconada L, Díaz-Fernández A, Ferapontova E E. Ultrasensitive disposable apatasensor for reagentless electrocatalytic detection of thrombin: An O₂-dependent hemin-G₄-aptamer assay on gold screen-printed electrodes[J]. Talanta, 2022,245(11)123456.
2. [2]
  Sun J F, Wang G X, Cheng H, Han Y F, Li Q, Jiang C. An antifouling electrochemical aptasensor based on hyaluronic acid functionalized polydopamine for thrombin detection in human serum[J]. Bioelectrochemistry, 2022,145108073. doi: 10.1016/j.bioelechem.2022.108073
3. [3]
  LI D L, GU M Q, WANG M, CHI K N, ZHANG X, DENG Y, MA Y C, HU R, YANG Y H. Preparation of thrombin aptasensor based on the metal‑organic framework Fe‑MIL‑88NH₂[J]. Chem. J. Chinese Universities, 2019,40(3):439-447.
4. [4]
  Huang Y, Zhao S L, Chen Z F, Shi M, Chen J, Liang H. An amplified chemiluminescence aptasensor based on bi-resonance energy transfer on gold nanoparticles and exonuclease Ⅲ-catalyzed target recycling[J]. Chem. Commun., 2012,48(97):11877-11879. doi: 10.1039/c2cc37130h
5. [5]
  Liao X J, Zhang C Y, Machuki achwa J O, Wen X Q, Tang Q L, Shi H L, Gao F L. Proximity hybridization-triggered DNA assembly for label-free surface-enhanced Raman spectroscopic bioanalysis[J]. Anal. Chim. Acta, 2020,1139(47):42-49.
6. [6]
  Cui H Y, Fu X Q, Yang L, Xing S, Wang X F. 2D titanium carbide nanosheets based fluorescent aptasensor for sensitive detection of thrombin[J]. Talanta, 2021,228(1)122219.
7. [7]
  Su J, Liu W H, Chen S X, Deng W P, Dou Y Z, Zhao Z H, Li J Y, Li Z H, Yin H, Ding X T, Song S P. A carbon-based DNA framework nano-bio interface for biosensing with high sensitivity and a high signal-to-noise ratio[J]. ACS Sens., 2020,5(12):3979-3987. doi: 10.1021/acssensors.0c01745
8. [8]
  Ma C, Cao Y, Gou X D, Jun J J. Recent progress in electrochemiluminescence sensing and imaging[J]. Anal. Chem., 2020,92(1):431-454. doi: 10.1021/acs.analchem.9b04947
9. [9]
  Blasi D, Sarcina L, Tricase A, Stefanachi A, Leonetti F, Alberga D, Mangiatordi G F, Manoli K, Scamarcio G, Picca R A, Torsi L. Enhancing the sensitivity of biotinylated surfaces by tailoring the design of the mixed self-assembled monolayer synthesis[J]. ACS Omega, 2020,5(27):16762-16771. doi: 10.1021/acsomega.0c01717
10. [10]
  Zheng Y N, Yuan Y L, Chai Y Q, Yuan R. L-cysteine induced manganese porphyrin electrocatalytic amplification with 3D DNA‑Au@Pt nanoparticles as nanocarriers for sensitive electrochemical aptasensor[J]. Biosens. Bioelectron., 2016,798:6-91.
11. [11]
  Hu G B, Xiong C Y, Liang W B, Yang Y, Yao L Y, Huang W, Luo W, Yuan R, Xiao D R. Highly stable Ru-complex-grafted 2D metal-organic layer with superior electrochemiluminescent efficiency as a sensing platform for simple and ultrasensitive detection of mucin 1[J]. Biosens. Bioelectron., 2019,135:95-101. doi: 10.1016/j.bios.2019.03.026
12. [12]
  Wang C, Han Q, Mo F J, Chen M, Xiong Z W, Fu Y Z. Novel luminescent nanostructured coordination polymer: Facile fabrication and application in electrochemiluminescence biosensor for microRNA-141 detection[J]. Anal. Chem., 2020,92(18):12145-12151. doi: 10.1021/acs.analchem.0c00130
13. [13]
  Zhang X X, Liao F S, Wang M, Zhang J, Xu B X, Zhang L, Xiong J, Xiong W. Enzyme-free recycling amplification-based sensitive electrochemical thrombin aptasensor[J]. Electroanalysis, 2021,33:1152-1159. doi: 10.1002/elan.202060496
14. [14]
  Chen Y, Li S B, Zhang L, Jing T, Wang J X, Zhao L J, Li F B, Li C, Sun J Y. Facile and fast synthesis of three-dimensional Ce-MOF/Ti₃C₂T_X MXene composite for high performance electrochemical sensing of L-tryptophan[J]. J. Solid. State. Chem., 2022,308122919. doi: 10.1016/j.jssc.2022.122919
15. [15]
  Zhang L, Sun M, Jing T, Li S B, Ma H Y. A facile electrochemical sensor based on green synthesis of Cs/Ce-MOF for detection of tryptophan in human serum[J]. Colloid. Surface A, 2022,648(5)129225.
16. [16]
  Tu X L, Xie Y, Ma X, Gao F, Gong L, Wang D W, Lu L M, Liu G B, Yu Y F, Huang X G. Highly stable reduced graphene oxide-encapsulated Ce-MOF composite as sensing material for electrochemically detecting dichlorophen[J]. J. Electroanal. Chem., 2019,848(1)113268.
17. [17]
  Huang H P, Chen Y N, Chen Z Z, Chen J L, Hu Y M, Zhu J J. Electrochemical sensor based on Ce-MOF/carbon nanotube composite for the simultaneous discrimination of hydroquinone and catechol[J]. J. Hazard. Mater., 2021,416(15)125895.
18. [18]
  Chen F, Wang Y M, Guo W W, Yin X B. Color-tunable lanthanide metal-organic framework gels[J]. Chem Sci., 2019,10(6):1644-1650. doi: 10.1039/C8SC04732D
19. [19]
  Jing P, Xu W J, Yi H Y, Wu Y M, Bai L J, Yuan R. An amplified electrochemical aptasensor for thrombin detection based on pseudobienzymic Fe₃O₄-Au nanocomposites and electroactive hemin/G-quadruplex as signal enhancers[J]. Analyst, 2014,139(7):1756-1761. doi: 10.1039/c3an02237d
20. [20]
  Shi J, Claussen J C, McLamore E S, Haque A U, Jaroch D, Diggs A R, Calvo-Marzal P, Rickus J L, Porterfield D M. A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors[J]. Nanotechnol., 2011,22355502. doi: 10.1088/0957-4484/22/35/355502
21. [21]
  Zhang J, Song S P, Wang L, H , Pan D, Fan C H. A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA[J]. Nat. Protoc., 2007,2:2888-2895. doi: 10.1038/nprot.2007.419
22. [22]
  Jiang J Z, Cai Q, Deng M H. Construction of electrochemical aptamer sensor based on Pt-coordinated titanium-based porphyrin MOF for thrombin detection[J]. Front. Chem., 2022,9812983. doi: 10.3389/fchem.2021.812983
23. [23]
  Zhang Q X, Fan G C, Chen W, Liu Q, Zhang X, Zhang X X, Liu Q Y. Electrochemical sandwich-type thrombin aptasensor based on dual signal amplification strategy of silver nanowires and hollow Au-CeO₂[J]. Biosens. Bioelectron., 2020,150111846. doi: 10.1016/j.bios.2019.111846
24. [24]
  Zhu J, Gan H Y, Wu J, Ju H X. Molecular machine powered surface programmatic chain reaction for highly sensitive electrochemical detection of protein[J]. Anal. Chem., 2018,90(8):5503-5508. doi: 10.1021/acs.analchem.8b01217
25. [25]
  Park K. Impedance technique-based label-free electrochemical aptasensor for thrombin using single-walled carbon nanotubes-casted screen-printed carbon electrode[J]. Sensors, 2022,22(7)2699. doi: 10.3390/s22072699
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