Citation: Cun WANG, Xiao-Chuan ZOU, Yuan-Bo HUANG, Jing HU, Ming-Chuan DENG, Xia FENG. Electrochemical aptamer sensor for thrombin based on cerium complex signal probe[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(10): 1931-1940. doi: 10.11862/CJIC.2023.162 shu

Electrochemical aptamer sensor for thrombin based on cerium complex signal probe

College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China

Corresponding author: Xiao-Chuan ZOU, zxcvip2003@163.com
Received Date: 3 January 2023
Revised Date: 17 September 2023

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In this work, a series of cerium coordination polymers (Ce-COPs) with different morphology and electro- chemical signals were synthesized by a simple hydrothermal method using Ce³⁺ as the central ion and N, N-dimethyl- formamide as the organic ligand through temperature regulation. The polyhedral Ce-COP with the largest electro- chemical signal was selected as the signal probe. Then, a sensitive thrombin (TB) aptamer sensor was designed through the specific recognition between TB and TB aptamer. Under the optimal experimental conditions, the linear range for TB detection was from 1.0 fmol·L^-1 to 1.0 nmol·L^-1, and the detection limit was 0.94 fmol·L^-1. In addition, our method is similar to the results of commercial human thrombin (TM) ELISA reagent detection. In a word, our biosensor possesses good sensitivity, specificity, selectivity, and stability.
- cerium coordination polymer,
- sensor,
- thrombin,
- aptamer,
- electrochemistry
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