Citation: Ying WANG, Rui XUE, Sheng-Li HUANG, Guo-Yu YANG. Luminescent heterobimetallic probes for selectively locating DNA in the nucleus and mitochondria of live cells[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(8): 1463-1470. doi: 10.11862/CJIC.2023.124 shu

Luminescent heterobimetallic probes for selectively locating DNA in the nucleus and mitochondria of live cells

MOE Key Laboratory of Cluster Science, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China

Corresponding author: Rui XUE, chemichem@163.com Sheng-Li HUANG, huangsl@bit.edu.cn
Received Date: 26 March 2023
Revised Date: 20 May 2023

Figures(4)

Nucleus probes for real-time imaging of DNA in live cells are very rare. Herein, the combined use of Ru(N^N)₃, Ir(C^N)₂(N^N), Re(CO)₃Cl(N^N), and Pt(C^N)(N^N) produced four heterobimetallic complexes. Two different functional metal sites are arranged at both ends of linear bis-chelating linkers (N^N)-(N^N), leading to asymmetric dumbbell-like molecules M₁-M₂, namely, Ru-Re, Ru-Pt, Ir-Re, and Ir-Pt. The red emissive probes of Ru-Re and Ru-Pt offer the benefits of large Stokes shift, excellent nucleus membrane penetration, and DNA binding ability with good photostability. Moreover, DNA imaging of Ru-Re and Ru-Pt probes was comparable to the commercial dye of Hoechst 33342 which is specially used for staining DNA in the nucleus of live cells. However, the Ir-based probes of Ir-Re and Ir-Pt directly targeted mitochondria. The emission properties and selective intracellular localizations of different probes are highly dependent on the metal species used in the heterobimetallic complexes.
- heterobimetallic probes,
- DNA imaging,
- mitochondria staining,
- cellular uptake
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