Citation: Zhen WANG, Si-Yuan LI, Yuan WANG, Wei-Na WU, Lei ZHANG, Zhong CHEN, Ling-Ling YAN. Pyrrole-based hydrazone for fluorescent imaging of Hg²⁺ in lysosomes[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(6): 1122-1130. doi: 10.11862/CJIC.2023.083 shu

Pyrrole-based hydrazone for fluorescent imaging of Hg²⁺ in lysosomes

Zhen WANG ¹ ,
Si-Yuan LI ² ,
Yuan WANG ¹ ,
Wei-Na WU ¹ ,
Lei ZHANG ^1,, ,
Zhong CHEN ^2,, ,
Ling-Ling YAN ³

1.
College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China
2.
School of Materials and Mechanical and Electrical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 China
3.
School of Physics and Electronic Information Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China

Corresponding author: Lei ZHANG, leizh2008@163.com Zhong CHEN, chenzhonglzu@hotmail.com
Received Date: 3 April 2022
Revised Date: 27 April 2023

Figures(9)

A novel pyrrole-based hydrazone has been synthesized for colorimetric and fluorescent turn-on detection of Hg²⁺ ions. A 1:1 binding ratio of the Hg²⁺ complex has been obtained from Job's plots and MS data. The coordination mode was systematically investigated by ¹H NMR, time-resolved fluorescence spectroscopy, and density functional theory (DFT) calculations. The limit of detection of 1 for Hg²⁺ was as low as 45 nmol·L^-1 with an association constant of 5.78×10⁸ L·mol^-1. It is worth noting that the response of 1 to Hg²⁺ was good in a pH range of 4.0 to 10.0. In addition, owing to the existence of the morpholine group, the probe can detect lysosomal Hg²⁺ in HeLa cells.
- cell imaging,
- fluorescent probe,
- Hg²⁺,
- hydrazone,
- lysosome,
- pyrrole
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Pyrrole-based hydrazone for fluorescent imaging of Hg2+ in lysosomes

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通讯作者: 陈斌, bchen63@163.com

Pyrrole-based hydrazone for fluorescent imaging of Hg²⁺ in lysosomes