Citation: Qing-Mei ZHANG, Lu-Peng ZHANG, Kai-Wen ZHENG, Guo-Qing YANG, Song-Jie HE, Xiu-Juan DU, Feng-Hua CHEN, Bing LI. Green synthesis of high-stability black rice carbon dots for application in cell imaging[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(4): 735-745. doi: 10.11862/CJIC.2023.045 shu

Green synthesis of high-stability black rice carbon dots for application in cell imaging

1.
School of Applied Science, Taiyuan University of Science and Technology, Taiyuan 030024, China
2.
Hospital of Stomatology, Shanxi Medical University, Taiyuan 030001, China

Corresponding author: Bing LI, Libing-1975@163.com
Received Date: 21 September 2022
Revised Date: 15 March 2023

Figures(6)

Carbon dots (CDs) were synthesized by a facile hydrothermal method using cheap black rice as a carbon source.The synthesized CDs exhibited excellent stability, which was not sensitive to changes in temperature, pH, and solution of NaCl, and were immune to many metal ions.The purified CDs also had low cytotoxicity against human oral keratinocytes (Hok), cancer cells (cervical cancer, Hela), and bone marrow mesenchymal stem (BMSC, MC3T3) cells.Meanwhile, it behaved with excitation-independent behavior in the shorter wavelength range (410-470 nm) and excitation-dependent behavior in the longer wavelength range (470-510 nm).Due to the excellent fluorescence properties and stability, we applied them to the intracellular imaging of Hok, Hela, and MC3T3 cells.
- black rice carbon dots,
- fluorescence properties,
- stability,
- cell imaging
1. [1]
  Gao X H, Du C, Zhuang Z H, Chen W. Carbon quantum dot -based nanoprobes for metal ion detection[J]. J. Mater. Chem. C, 2016,4:6927-6945. doi: 10.1039/C6TC02055K
2. [2]
  Tejwan N, Saha S K, Das J. Multifaceted applications of green carbon dots synthesized from renewable sources[J]. Adv. Colloid Interface Sci., 2020,275102046. doi: 10.1016/j.cis.2019.102046
3. [3]
  ZHU Z C, YANG B. Fabrication and application of fluorescence biosensors based on carbon dots and aptamer[J]. Chinese Journal of Luminescence, 2021,42(8):1196-1214. doi: 10.37188/CJL.20210161
4. [4]
  Li H X, Yan X, Kong D S, Jin R, Sun C Y, Du D, Lin Y H, Lu G Y. Recent advances in carbon dots for bioimaging applications[J]. Nanoscale Horiz., 2020,5:218-234. doi: 10.1039/C9NH00476A
5. [5]
  Ding H, Zhou X X, Wei J S, Li X B, Qin B T, Chen X B, Xiong H M. Carbon dots with red/near-infrared emissions and their intrinsic merits for biomedical applications[J]. Carbon, 2020,167:322-344. doi: 10.1016/j.carbon.2020.06.024
6. [6]
  Chung Y J, Kim J, Park C B. Photonic carbon dots as an emerging nanoagent for biomedical and health care applications[J]. ACS Nano, 2020,14:6470-6497. doi: 10.1021/acsnano.0c02114
7. [7]
  ZHENG M, LIU K M, SU Y. Carbon dots for biomedical application[J]. Chinese Journal of Luminescence, 2021,42(8):1233-1244.
8. [8]
  MAO H H, ZHAN Z H, ZHOU G H, XUE M Y, HAN G C, JIANG M X, LUO X N, LIN X Y. Advances in application of fluorescent carbon quantum dots in drug analysis[J]. Chinese Journal of Luminescence, 2021,42(8):1245-1256.
9. [9]
  Tian Z, Zhang X T, Li D, Zhou D, Jing P T, Shen D Z, Qu S N, Zboril R, Rogach A L. Full-color inorganic carbon dot phosphors for whitelight-emitting diodes[J]. Adv. Opt. Mater., 2017,51700416. doi: 10.1002/adom.201700416
10. [10]
  Yuan F L, Yuan T, Sui L Z, Wang Z B, Xi Z F, Li Y C, Li X H, Fan L Z, Tan Z A, Chen A M, Jin M X, Yang S H. Engineering triangular carbon quantum dots with unprecedented narrow bandwidth emission for multicolored LEDs[J]. Nat. Commun., 2018,92249. doi: 10.1038/s41467-018-04635-5
11. [11]
  WANG Q, YANG W, ZHUANG J R, LI J, YANG P Z, WANG Z L. Preparation and application of bio-based carbon dots for LED chips[J]. Chinese Journal of Luminescence, 2021,42(8):1314-1322. doi: 10.37188/CJL.20210159
12. [12]
  Gao S Y, Chen Y L, Fan H, Wei X J, Hu C G, Wang L X, Qu L T. A green one-arrow-two-hawks strategy for nitrogen-doped carbon dots as fluorescent ink and oxygen reduction electrocatalysts[J]. J. Mater. Chem. A, 2014,2:6320-6325. doi: 10.1039/c3ta15443b
13. [13]
  Zhang J, Yuan Y, Liang G L, Yu S H. Scale-up synthesis of fragrant nitrogen -doped carbon dots from bee pollens for bioimaging and catalysis[J]. Adv. Sci., 2015,21500002. doi: 10.1002/advs.201500002
14. [14]
  Guo J Z, Li H, Ling L T, Li G, Cheng R, Lu X, Xie A Q, Li Q, Wang C F, Chen S. Green synthesis of carbon dots toward anti-counterfeiting[J]. ACS Sustain. Chem. Eng., 2019,8:1566-1572.
15. [15]
  LIU J, ZHANG X R, XIONG H M. Application of fluorescent carbon dots in fingerprint detection[J]. Chinese Journal of Luminescence, 2021,42(8):1095-1113.
16. [16]
  Permatasari F A, Fukazawa H, Ogi T, Iskandar F, Okuyama K. Design of pyrrolic-N -rich carbon dots with absorption in the first nearinfrared window for photothermal therapy[J]. ACS Appl. Nano Mater., 2018,1:2368-2375. doi: 10.1021/acsanm.8b00497
17. [17]
  NAN F C, XUE X K, GE J C, WANG P F. Recent advances of red/near infrared light responsive carbon dots for tumor therapy[J]. Chinese Journal of Luminescence, 2021,42(8):1155-1171.
18. [18]
  Alam A M, Park B Y, Ghouri Z K, Park M, Kim H Y. Synthesis of carbon quantum dot from cabbage with down -and up -conversion photoluminescence properties: Excellent imaging agent for biomedical application[J]. Green Chem., 2015,17:3791-3797. doi: 10.1039/C5GC00686D
19. [19]
  Zhao S J, Lan M H, Zhu X Y, Xue H T, Ng T W, Meng X M, Lee C S, Wang P F, Zhang W J. Green synthesis of bifunctional fluorescent carbon dots from garlic for cellular imaging and free radical scavenging[J]. ACS Appl. Mater. Interfaces, 2015,7:17054-17060. doi: 10.1021/acsami.5b03228
20. [20]
  Liu Y, Liu Y N, Park M, Park S J, Zhang Y F, Akanda M R, Park B Y, Kim H Y. Green synthesis of fluorescent carbon dots from carrot juice for in vitro cellular imaging[J]. Carbon Lett., 2017,21:61-67. doi: 10.5714/CL.2017.21.061
21. [21]
  Jeong C J, Roy A K, Kim S H, Lee J E, Jeong J H, In I, Park S Y. Fluorescence carbon nanoparticles derived from natural material of mango fruit for bio-imaging probes[J]. Nanoscale, 2014,6:15196-15202. doi: 10.1039/C4NR04805A
22. [22]
  Yang R, Guo X F, Jia L H, Zhang Y, Zhao Z L, Lonshakov F. Green preparation of carbon dots with mangosteen pulp for the selective detection of Fe³⁺ ions and cell imaging[J]. Appl. Surf. Sci., 2017,423:426-432. doi: 10.1016/j.apsusc.2017.05.252
23. [23]
  HU Y F, ZHANG L L, LIN L Y, LI X F, ZHAO S L, LIANG H. Preparation of carbon quantum dots from lycium chinensis and as a fluorescent probe for high sensitive detection of D -penicillamine[J]. Scientia Sinica Chimica, 2016,47:258-266.
24. [24]
  Konwar A, Chowdhury D. Property relationship of Alginate and Alginate-carbon dot nanocomposite with bivalent and trivalent crosslinker ions[J]. RSC Adv., 2015,5:62864-62870. doi: 10.1039/C5RA09887D
25. [25]
  Wang Z Y, Liao H, Wu H, Wang B B, Zhao H D, Tan M Q. Fluorescent carbon dots from beer for breast cancer cell imaging and drug delivery[J]. Anal. Methods, 2015,7:8911-8917. doi: 10.1039/C5AY01978H
26. [26]
  Li W D, Liu Y, Wu M, Feng X L, Redfern S A T, Shang Y, Yong X, Feng T L, Wu K F, Liu Z Y, Li B J, Chen Z M, Tse J S, Lu S Y, Yang B. Carbon-quantum-dots-loaded ruthenium nanoparticles as an efficient electrocatalyst for hydrogen production in alkaline media[J]. Adv. Mater., 2018,30e1800676. doi: 10.1002/adma.201800676
27. [27]
  Song Y, Yan X, Li Z H, Qu N B, Zhu C Z, Ye R F, Li S Q, Du D, Lin Y H. Highly photoluminescent carbon dots derived from linseed and their applications in cellular imaging and sensing[J]. J. Mater. Chem B, 2018,6:3181-3187. doi: 10.1039/C8TB00116B
28. [28]
  Yu C Y, Xuan T T, Chen Y W, Zhao Z J, Sun Z, Li H L. A facile, green synthesis of highly fluorescent carbon nanoparticles from oatmeal for cell imaging[J]. J. Mater. Chem. C, 2015,3:9514-9518. doi: 10.1039/C5TC02057C
29. [29]
  Zhang Y, Gao Z Y, Yang X, Chang J L, Liu Z Y, Jiang K. Fish-scalederived carbon dots as efficient fluorescent nanoprobes for detection of ferric ions[J]. RSC Adv., 2019,9:940-949.
30. [30]
  Sawalha S, Silvestri A, Criado A, Bettini S, Prato M, Valli L. Tailoring the sensing abilities of carbon nanodots obtained from olive solid wastes[J]. Carbon, 2020,167:696-708. doi: 10.1016/j.carbon.2020.06.011
31. [31]
  Meng W X, Bai X, Wang B Y, Liu Z Y, Lu S Y, Yang B. Biomassderived carbon dots and their applications[J]. Energy Environ. Mater., 2019,2:172-192.
32. [32]
  Li L P, Zhang R P, Lu C X, Sun J H, Wang L J, Qu B T, Li T T, Liu Y D, Li S J. In situ synthesis of NIR -light emission carbon dots derived from spinach for bio-imaging application[J]. J. Mater. Chem. B, 2017,5:7328-7334.
33. [33]
  Liao J, Cheng Z H, Zhou L. Nitrogen -doping enhanced fluorescent carbon dots: Green synthesis and their applications for bioimaging and label -free detection of Au³⁺ ions[J]. ACS Sustain. Chem. Eng., 2016,4:3053-3061.
34. [34]
  Krysmann M J, Kelarakis A, Dallas P, Giannelis E P. Formation mechanism of carbogenic nanoparticles with dual photoluminescence emission[J]. J. Am. Chem. Soc., 2012,134:747-750.
35. [35]
  Li L L, Wu G H, Yang G H, Peng J, Zhao J W, Zhu J J. Focusing on luminescent graphene quantum dots: Current status and future perspectives[J]. Nanoscale, 2013,5:4015-4039.
36. [36]
  Liu W, Diao H P, Chang H H, Wang H J, Li T T, Wei W L. Green synthesis of carbon dots from rose -heart radish and application for Fe³⁺ detection and cell imaging[J]. Sens. Actuators B-Chem., 2017,241:190-198.
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