Citation: Chang-Li ZHANG, Jing-Jing ZHANG, You SHEN, Jia-Cheng LU, Fang HUANG, Li XU. A Fas-Responsive Mitochondria-Targeting Fluorescent Probe Detecting Hypochlorite in Living Cells and Zebrafish[J]. Chinese Journal of Inorganic Chemistry, ;2022, 38(8): 1623-1632. doi: 10.11862/CJIC.2022.168 shu

A Fas-Responsive Mitochondria-Targeting Fluorescent Probe Detecting Hypochlorite in Living Cells and Zebrafish

1.
Key Laboratory of Advanced Functional Materials of Nanjing, School of Environmental Science, Nanjing Xiaozhuang University, Nanjing 211171, China
2.
College of Science, Nanjing Forestry University, Nanjing 210093, China

Corresponding author: Chang-Li ZHANG, zhangcl@njxzc.edu.cn; xuliqby@njfu.edu.cn Li XU, xuliqby@njfu.edu.cn
Received Date: 11 February 2022
Revised Date: 9 May 2022

Figures(6)

Herein, a mitochondria-targeting fluorescent probe Cou-Py has been designed and synthesized for sensing hypochlorite (ClO^-). Cou-Py exhibited very weak fluorescence due to the C=N isomerization of the oxime group in the excited state and recovers fluorescence within 5 s based on a ClO^--triggered deoximation reaction. Additionally, Cou-Py showed high selectivity toward ClO^- over other reactive oxygen species (ROS) and owned a low detection limit (6.87 nmol·L^-1) for ClO^-. Importantly, Cou-Py has been successfully employed for visualizing ClO^- in the mitochondria of MCF-7 cells as well as zebrafish larvae.
- fluorescent targeting probe,
- hypochlorite,
- zebrafish imaging,
- coumarin
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