Citation: Jing REN, Jin-Hui YAN, An-Yi ZHANG, Yu-Xing YAN, Cun-Cun WANG, Lin LI. pH Regulated Nanomedicine Based on Y-Type Molecular Sieve Loading Doxorubicin: Preparation and Interaction with MM-231 Cells[J]. Chinese Journal of Inorganic Chemistry, ;2022, 38(1): 93-102. doi: 10.11862/CJIC.2022.019 shu

pH Regulated Nanomedicine Based on Y-Type Molecular Sieve Loading Doxorubicin: Preparation and Interaction with MM-231 Cells

Jing REN ^1,, ,
Jin-Hui YAN ¹ ,
An-Yi ZHANG ¹ ,
Yu-Xing YAN ¹ ,
Cun-Cun WANG ¹ ,
Lin LI ^{1, 2,,}

1.
Department of Chemistry, Taiyuan Normal University, Jinzhong, Shanxi 030619, China
2.
Humic Acid Engineering and Technology Research Center of Shanxi Province, Jinzhong, Shanxi 030619, China

Corresponding author: Jing REN, jing2008_1231@126.com Lin LI, lilin@tynu.edu.cn
Received Date: 14 July 2021
Revised Date: 19 October 2021

Figures(12)

To overcome the shortcomings of poor antitumor drug targeting, low efficacy, and high toxicity, a pH regulated drug delivery system (YMS-DOX) through hydrogen bonds and van der Waals force was constructed to acquire high drug loading capacity and better therapeutic efficiency based on Y-type molecular sieve (YMS) as nano-carrier and doxorubicin (DOX) as the model drug. The successful formation of YMS-DOX was confirmed by using UV-Vis, FT-IR, particle size and potential measurement, and fluorescence spectroscopy. Interestingly, with the assistance of citric acid buffer solution (pH=9.0), YMS nanocarrier loading DOX achieved nearly 99.61% loading efficiency. In vitro drug release showed that YMS had low premature drug release under physiological conditions (pH=7.4), while greatly enhanced in the tumor microenvironment (pH=4.5) 3.8 times compared to normal tissue, indicating YMSDOX can be effectively released in the tumor site. Excitingly, when dendritic (DC) cells and breast cancer (MM -231) cells were treated with YMS-DOX, the results demonstrated that YMS-DOX preferentially accumulated much more in tumor cells than in normal cells, which implies that YMS-DOX can selectively kill tumor cells. In addition, assessment by flow cytometry apoptosis assay illustrated that YMS-DOX could induce cell apoptosis and inhibit cell migration.
- Y-type molecular sieve,
- doxorubicin,
- pH regulating,
- high loading,
- breast cancer therapy
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