Citation: Xiao-Ting QIAN, Meng-Di LÜ, Qun WANG, Qin TAO, Xu-Ling XUE, Hong-Ke LIU. Synthesis and Antitumor Properties of 3-(2-Pyridine-3-vinyl)-1H-indole Dipyridine Ruthenium Complex[J]. Chinese Journal of Inorganic Chemistry, ;2021, 37(5): 824-834. doi: 10.11862/CJIC.2021.099 shu

Synthesis and Antitumor Properties of 3-(2-Pyridine-3-vinyl)-1H-indole Dipyridine Ruthenium Complex

School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China

Corresponding author: Xu-Ling XUE, xuexuling87@163.com Hong-Ke LIU, liuhongke@njnu.edu.cn
Received Date: 13 November 2020
Revised Date: 5 January 2021

Figures(6)

In this work, we designed a novel bipyridine ruthenium complex Ru-Indole using 3-(2-pyridine-3-vinyl)¹H-indoles (Indole) as the ligand with the bipyridine ruthenium precursor Ru(bpy)₂Cl₂ as the coordination linkage. The structures of ligand and related complexes were characterized by using ¹H NMR, ESI-MS and elemental analysis. Using a fluorescence spectrophotometer and an ultraviolet-visible spectrophotometer, it is found that the complex could emit fluorescence under the excitation of UV-Vis light, which realized the visual imaging of the complex in the cell. The introduction of the ligand greatly improved the lipophilicity of the complex Ru-Indole, rendering it to be easier to enter the cell and exhibited better anti tumor activity compared with the ligand Indole and the precursor Ru(bpy)₂Cl₂. Furthermore, we used flow cytometry, confocal imaging and western blotting to explore the cell death mechanism induced by Ru-Indole. The results show that the complex Ru-Indole could be enriched in the mitochondria and lysosomes of tumor cells, and could change the mitochondrial membrane potential and at last induce the autophagy.
- bipyridine ruthenium complex,
- indole derivatives,
- antitumor,
- mitochondrial pathway,
- autophagy
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