Citation: HUANG Fang, ZHANG Chong-Yu, HUANG Ling-Hong, OUYANG Jian-Ming. Inhibition of Diethyl Citrate, Sodium Citrate, and Phosphonoformic Acid on Mouse Aortic Smooth Muscle Cells Calcification Induced by High Calcium[J]. Chinese Journal of Inorganic Chemistry, ;2020, 36(7): 1353-1365. doi: 10.11862/CJIC.2020.155 shu

Inhibition of Diethyl Citrate, Sodium Citrate, and Phosphonoformic Acid on Mouse Aortic Smooth Muscle Cells Calcification Induced by High Calcium

Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: OUYANG Jian-Ming, toyjm@jnu.edu.cn
Received Date: 23 November 2019
Revised Date: 9 May 2020

Figures(8)

Mouse aortic smooth muscle cells (MOVAS) were cultured for 14 d under the conditions of high calcium and different concentrations of diethyl citrate (Et₂Cit), sodium citrate (Na₃Cit), and phosphonoformic acid (PFA). Alizarin red staining, immunofluorescence and annexin V staining were used to detect cell transdifferentiation, apoptosis, and cell calcium deposition. The inhibitory effects of Et₂Cit, Na₃Cit, and PFA on calcium-induced calcification of MOVAS cells and their possible mechanisms were studied. The results showed that Et₂Cit, Na₃Cit, and PFA can all reduce MOVAS calcification induced by high calcium and reduce extracellular calcified plaque and calcium deposition. These inhibitors can inhibit the transdifferentiation of MOVAS to osteoblast-like cells, resulting in a decrease in the activity of alkaline phosphatase (ALP), a marker transformed to osteogenic differentiation. Their inhibitory effects exhibited concentration dependence. The inhibitory effects of the inhibitors at the same concentration showed the following trend:PFA > Na₃Cit > Et₂Cit. Low concentrations of Na₃Cit and Et₂Cit inhibit calcification by reducing cell apoptosis, but high concentrations of Na₃Cit, Et₂Cit, and PFA increased cell apoptosis due to their own toxicity. Et₂Cit and Na₃Cit as blood anticoagulants can effectively inhibit MOVAS calcification induced by high calcium.
- biomineralization,
- calcium phosphate,
- vascular calcification,
- high calcium,
- diethyl citrate,
- sodium citrate,
- phosphonoformic acid
1. [1]
  Voelkl J, Cejka D, Alesutan I. Curr. Opin. Nephrol. Hypertens., 2019, 28(4):289-296 doi: 10.1097/MNH.0000000000000507
2. [2]
  Durham A L, Speer M Y, Scatena M, et al. Cardiovasc. Res., 2018, 114(4):590-600 doi: 10.1093/cvr/cvy010
3. [3]
  Voelkl J, Lang F, Eckardt K U, et al. Cell. Mol. Life Sci., 2019, 76(11):2077-2091
4. [4]
  Lomashvili K A, Wang X, Wallin R, et al. J. Biol. Chem., 2011, 286(33):28715-28722 doi: 10.1074/jbc.M111.251462
5. [5]
  Ou Y, Han J, Chen B, et al. Asian J. Chem., 2012, 24(11):4953-4960
6. [6]
  Kose N, Aytacoglu B N, Yilmaz N, et al. Anadolu Kardiyol. Derg., 2008, 8(2):94-98
7. [7]
  Hénaut L, Boudot C, Massy Z A, et al. Cardiovasc. Res., 2014, 101(2):256-265 doi: 10.1093/cvr/cvt249
8. [8]
  Masumoto A, Sonou T, Ohya M. J. Atheroscler. Thromb., 2017, 24(7):716-724 doi: 10.5551/jat.36574
9. [9]
  Louvet L, Büchel J, Steppan S, et al. Nephrol. Dial. Transplant., 2013, 28(4):869-878 doi: 10.1093/ndt/gfs520
10. [10]
  Li M C, Wu P F, Shao J, et al. Cardiovasc. Toxicol., 2016, 16(2):172-181
11. [11]
  Cai L, Li W, Wang G W, et al. Diabetes, 2002, 51(6):19381948
12. [12]
  Villa-Bellosta R, Sorribas V. Arterioscler. Thromb. Vasc. Biol., 2009, 29(5):761-766 doi: 10.1161/ATVBAHA.108.183384
13. [13]
  Tani T, Fujiwara M, Orimo H, et al. J. Pathol., 2020, 250(1):30-41
14. [14]
  Lau W L, Festing M, Giachelli C M. Thromb. Haemostasis, 2010, 104(3):464-470
15. [15]
  Yu W, Zhuang F, Ma S, et al. Nephron, 2019, 141(2):119-127 doi: 10.1159/000494693
16. [16]
  Meng Y, Zhang H, Li Y B, et al. Bone, 2014, 58:168-176 doi: 10.1016/j.bone.2013.10.010
17. [17]
  Truss N J, Armstrong P C J, et al. Thromb. Haemostasis, 2009, 7(11):1897-1905 doi: 10.1111/j.1538-7836.2009.03589.x
18. [18]
  Clark J A, Schulman G, Golper T A. Clin. J. Am. Soc. Nephrol., 2008, 3(3):736-742 doi: 10.2215/CJN.03460807
19. [19]
  Ou Y, Chen B, Peng H, et al. Asian J. Chem., 2012, 24(10):4717-4722
20. [20]
  Han J, Xue J F, Xu M, et al. Bioinorg. Chem. Appl., 2013, 354736:1-8
21. [21]
  Chen Z, Chen B, Yao X Q, et al. Blood Purif., 2012, 33(1):3036
22. [22]
  Liu Y R, Zhang L, Ni Z H, et al. Calcified Tissue Int., 2016, 99(5):543-555 doi: 10.1007/s00223-016-0182-y
23. [23]
  Azpiazu D, Gonzalo S, González-Parra E, et al. Nefrologia, 2018, 38(3):250-257 doi: 10.1016/j.nefro.2017.07.005
24. [24]
  Bisson S K, Ung R V, Picard S, et al. J. Bone Miner. Metab., 2019, 37(2):212-223 doi: 10.1007/s00774-018-0919-y
25. [25]
  Hu Y Y, Rawal Y, Schmidt-Rohr K. Proc. Natl. Acad. Sci. U. S.A., 2010, 107(52):22425-22429 doi: 10.1073/pnas.1009219107
26. [26]
  Rimer J D, Sakhaee K, Maalouf N M. Curr. Opin. Nephrol. Hypertens., 2019, 28(2):130-139 doi: 10.1097/MNH.0000000000000474
27. [27]
  Hu Y Y, Liu X P, Ma X, et al. Chem. Mater., 2011, 23(9):2481-2490 doi: 10.1021/cm200355n
28. [28]
  Sheen C R, Kuss P, Narisawa S, et al. J. Bone Miner. Res., 2015, 30(5):824-836 doi: 10.1002/jbmr.2420
29. [29]
  Goettsch C, Hutcheson J D, Aikawa M, et al. J. Clin. Invest., 2016, 126(4):1323-1336 doi: 10.1172/JCI80851
30. [30]
  Braake A D, Tinnemans P T, Shanahan C M, et al. Sci. Rep., 2018, 8(1):2069
31. [31]
  Kapustin A N, Shanahan C M. Trends Cardiovasc. Med., 2012, 22(5):133-137 doi: 10.1016/j.tcm.2012.07.009
32. [32]
  Shroff R C, McNair R, Figg N, et al. Circulation, 2008, 118(17):1748-1757 doi: 10.1161/CIRCULATIONAHA.108.783738
33. [33]
  Wesseling-Perry K, Wang H J, Elashoff R, et al. J. Clin. Endocrinol. Metab., 2014, 99(10):E1951-E1956 doi: 10.1210/jc.2014-2125
34. [34]
  Ciceri P, Elli F, Braidotti P, et al. Atherosclerosis, 2016, 254:93-101 doi: 10.1016/j.atherosclerosis.2016.09.071
35. [35]
  Lu Y F, Zhang X D, Zhang H T, et al. Anticancer Res., 2011, 31(3):797-805
36. [36]
  Zavaczki E, Jeney V, Agarwal A, et al. Kidney Int., 2011, 80(7):731-739 doi: 10.1038/ki.2011.212
37. [37]
  Duan C Y, Zhang C Y, Sun X Y, et al. J. Cardiovasc. Pharmacol., 2017, 70(6):411-419 doi: 10.1097/FJC.0000000000000537
38. [38]
  Chang Y M, Tsai S C, Shiao C C, et al. Clin. Exp. Nephrol., 2017, 21(5):908-916
39. [39]
  Peter W L S, Wazny L D, Weinhandl E D. Am. J. Kidney Dis., 2018, 71(2):246-253
40. [40]
  Hortells L, Sosa C, Millán Á, et al. PloS One, 2015, 10(11):e0141751 doi: 10.1371/journal.pone.0141751
1. [1]
  Shiyu Pan , Bo Cao , Deling Yuan , Tifeng Jiao , Qingrui Zhang , Shoufeng Tang . Complexes of cupric ion and tartaric acid enhanced calcium peroxide Fenton-like reaction for metronidazole degradation. Chinese Chemical Letters, 2024, 35(7): 109185-. doi: 10.1016/j.cclet.2023.109185
2. [2]
  Yuhan Wu , Qing Zhao , Zhijie Wang . Layered vanadium oxides: Promising cathode materials for calcium-ion batteries. Chinese Journal of Structural Chemistry, 2024, 43(5): 100271-100271. doi: 10.1016/j.cjsc.2024.100271
3. [3]
  Xinyu You , Xin Zhang , Shican Jiang , Yiru Ye , Lin Gu , Hexun Zhou , Pandong Ma , Jamal Ftouni , Abhishek Dutta Chowdhury . Efficacy of Ca/ZSM-5 zeolites derived from precipitated calcium carbonate in the methanol-to-olefin process. Chinese Journal of Structural Chemistry, 2024, 43(4): 100265-100265. doi: 10.1016/j.cjsc.2024.100265
4. [4]
  Yi Cao , Xiaojiao Ge , Yuanyuan Wei , Lulu He , Aiguo Wu , Juan Li . Tumor microenvironment-activatable neuropeptide-drug conjugates enhanced tumor penetration and inhibition via multiple delivery pathways and calcium deposition. Chinese Chemical Letters, 2024, 35(4): 108672-. doi: 10.1016/j.cclet.2023.108672
5. [5]
  Xiping Dong , Xuan Wang , Zhixiu Lu , Qinhao Shi , Zhengyi Yang , Xuan Yu , Wuliang Feng , Xingli Zou , Yang Liu , Yufeng Zhao . Construction of Cu-Zn Co-doped layered materials for sodium-ion batteries with high cycle stability. Chinese Chemical Letters, 2024, 35(5): 108605-. doi: 10.1016/j.cclet.2023.108605
6. [6]
  Zhijia Zhang , Shihao Sun , Yuefang Chen , Yanhao Wei , Mengmeng Zhang , Chunsheng Li , Yan Sun , Shaofei Zhang , Yong Jiang . Epitaxial growth of Cu_2-xSe on Cu (220) crystal plane as high property anode for sodium storage. Chinese Chemical Letters, 2024, 35(7): 108922-. doi: 10.1016/j.cclet.2023.108922
7. [7]
  Shengyu Zhao , Xuan Yu , Yufeng Zhao . A water-stable high-voltage P3-type cathode for sodium-ion batteries. Chinese Chemical Letters, 2024, 35(9): 109933-. doi: 10.1016/j.cclet.2024.109933
8. [8]
  Luyan Shi , Ke Zhu , Yuting Yang , Qinrui Liang , Qimin Peng , Shuqing Zhou , Tayirjan Taylor Isimjan , Xiulin Yang . Phytic acid-derivative Co₂B-CoPO_x coralloidal structure with delicate boron vacancy for enhanced hydrogen generation from sodium borohydride. Chinese Chemical Letters, 2024, 35(4): 109222-. doi: 10.1016/j.cclet.2023.109222
9. [9]
  Guangchang Yang , Shenglong Yang , Jinlian Yu , Yishun Xie , Chunlei Tan , Feiyan Lai , Qianqian Jin , Hongqiang Wang , Xiaohui Zhang . Regulating local chemical environment in O3-type layered sodium oxides by dual-site Mg²⁺/B³⁺ substitution achieves durable and high-rate cathode. Chinese Chemical Letters, 2024, 35(9): 109722-. doi: 10.1016/j.cclet.2024.109722
10. [10]
  Yuxin Wang , Zhengxuan Song , Yutao Liu , Yang Chen , Jinping Li , Libo Li , Jia Yao . Methyl functionalization of trimesic acid in copper-based metal-organic framework for ammonia colorimetric sensing at high relative humidity. Chinese Chemical Letters, 2024, 35(6): 108779-. doi: 10.1016/j.cclet.2023.108779
11. [11]
  Tao Long , Peng Chen , Bin Feng , Caili Yang , Kairong Wang , Yulei Wang , Can Chen , Yaping Wang , Ruotong Li , Meng Wu , Minhuan Lan , Wei Kong Pang , Jian-Fang Wu , Yuan-Li Ding . Reinforced concrete-like Na_3.5V_1.5Mn_0.5(PO₄)₃@graphene hybrids with hierarchical porosity as durable and high-rate sodium-ion battery cathode. Chinese Chemical Letters, 2024, 35(4): 109267-. doi: 10.1016/j.cclet.2023.109267
12. [12]
  Lingna Wang , Chenxin Tian , Ruobin Dai , Zhiwei Wang . Eco-friendly regeneration of end-of-life PVDF membrane with triethyl phosphate: Efficiency and mechanism. Chinese Chemical Letters, 2024, 35(9): 109356-. doi: 10.1016/j.cclet.2023.109356
13. [13]
  Xinxiu Yan , Xizhe Huang , Yangyang Liu , Weishang Jia , Hualin Chen , Qi Yao , Tao Chen . Hyperbranched polyamidoamine protective layer with phosphate and carboxyl groups for dendrite-free Zn metal anodes. Chinese Chemical Letters, 2024, 35(10): 109426-. doi: 10.1016/j.cclet.2023.109426
14. [14]
  Ze-Yuan Ma , Mei Xiao , Cheng-Kun Li , Adedamola Shoberu , Jian-Ping Zou . S-(1,3-Dioxoisoindolin-2-yl)O,O-diethyl phosphorothioate (SDDP): A practical electrophilic reagent for the phosphorothiolation of electron-rich compounds. Chinese Chemical Letters, 2024, 35(5): 109076-. doi: 10.1016/j.cclet.2023.109076
15. [15]
  Haiying Lu , Weijie Li . The electrolyte solvation and interfacial chemistry for anode-free sodium metal batteries. Chinese Journal of Structural Chemistry, 2024, 43(11): 100334-100334. doi: 10.1016/j.cjsc.2024.100334
16. [16]
  Shengyu Zhao , Qinhao Shi , Wuliang Feng , Yang Liu , Xinxin Yang , Xingli Zou , Xionggang Lu , Yufeng Zhao . Suppression of multistep phase transitions of O3-type cathode for sodium-ion batteries. Chinese Chemical Letters, 2024, 35(5): 108606-. doi: 10.1016/j.cclet.2023.108606
17. [17]
  Fabrice Nelly Habarugira , Ducheng Yao , Wei Miao , Chengcheng Chu , Zhong Chen , Shun Mao . Synergy of sodium doping and nitrogen defects in carbon nitride for promoted photocatalytic synthesis of hydrogen peroxide. Chinese Chemical Letters, 2024, 35(8): 109886-. doi: 10.1016/j.cclet.2024.109886
18. [18]
  Mingxin Song , Lijing Xie , Fangyuan Su , Zonglin Yi , Quangui Guo , Cheng-Meng Chen . New insights into the effect of hard carbons microstructure on the diffusion of sodium ions into closed pores. Chinese Chemical Letters, 2024, 35(6): 109266-. doi: 10.1016/j.cclet.2023.109266
19. [19]
  Jun-Ming Cao , Kai-Yang Zhang , Jia-Lin Yang , Zhen-Yi Gu , Xing-Long Wu . Differential bonding behaviors of sodium/potassium-ion storage in sawdust waste carbon derivatives. Chinese Chemical Letters, 2024, 35(4): 109304-. doi: 10.1016/j.cclet.2023.109304
20. [20]
  Tao LIU , Yuting TIAN , Ke GAO , Xuwei HAN , Ru'nan MIN , Wenjing ZHAO , Xueyi SUN , Caixia YIN . A photothermal agent with high photothermal conversion efficiency and high stability for tumor therapy. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1622-1632. doi: 10.11862/CJIC.20240107

Get Citation

PDF

XML

Metrics

PDF Downloads(5)
Abstract views(334)
HTML views(68)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142