Citation: CHEN Bang, WANG Shao-Jing, SONG Zhan-Ke, GUO Yuan. Naphthol-Based Shiff Base as a Selective Fluorescent Probe for Detecting Zn²⁺ in Living Cells[J]. Chinese Journal of Inorganic Chemistry, ;2017, 33(10): 1722-1730. doi: 10.11862/CJIC.2017.227 shu

Naphthol-Based Shiff Base as a Selective Fluorescent Probe for Detecting Zn²⁺ in Living Cells

Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China

Corresponding author: GUO Yuan, guoyuan@nwu.edu.cn
Received Date: 23 May 2017
Revised Date: 1 September 2017

Figures(11)

Two fluorescent probes BMO and NBMO were developed for the highly selective detection of Zn²⁺ based on C=N isomerization and chelation-enhanced fluorescence (CHEF) mechanism. Structure identification of the probes was confirmed by ¹H NMR, ¹³C NMR, ¹H-¹H COSY, HSQC, IR and HRMS spectroscopy. The results of spectral analysis show that BMO and NBMO are sensitive and highly selective to Zn²⁺. The detection limit of BMO and NBMO are found to be 30 and 21 nmol·L^-1 respectively. There is a good linear relationship between the fluorescence intensity of probes (BMO and NBMO) and the concentration of Zn²⁺ in the range of 0 to 20 μmol·L^-1. The X-ray crystal structure of the NBMO-Zn²⁺ complex exposes its coordination feature and the Job plots reveal a 1:1 probe-Zn²⁺ identification. The cell images show that NBMO can be used to detect intracellular Zn²⁺.
- fluorescent probe,
- C=N isomerization,
- chelation-enhanced fluorescence,
- zinc ion,
- living cell imaging
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Naphthol-Based Shiff Base as a Selective Fluorescent Probe for Detecting Zn2+ in Living Cells

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通讯作者: 陈斌, bchen63@163.com

Naphthol-Based Shiff Base as a Selective Fluorescent Probe for Detecting Zn²⁺ in Living Cells