Citation: YANG Yang, HUO Wen-Shan, ZHOU Zheng, ZHANG Qi, ZENG Han. Direct Electrochemistry of Electrode Modified with Thin Film of Laccase Immobilized in Nano-Composite of Polyaniline-CoC₂O₄[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(12): 2117-2128. doi: 10.11862/CJIC.2016.277 shu

Direct Electrochemistry of Electrode Modified with Thin Film of Laccase Immobilized in Nano-Composite of Polyaniline-CoC₂O₄

1.
Laboratory of New Energy Materials Chemistry, Chemistry and Chemical Engineering Academy, XinJiang Normal University, Urumuqi 830054, China

Corresponding author: ZENG Han,
Received Date: 13 June 2016
Available Online: 14 October 2016
Fund Project:

Electrochemical methods including cyclic voltammetry, differential pulse voltammetry, electrochemical impedance spectrometry and chronoamperometry, together with auxiliary means such as F-T infrared spectrometry, Ultra-violet visible spectrometry, atomic force microscopy, transmission electron microscopy and atomic adsorption spectrometry were used to characterize the chemical composition, structure and morphology of polyaniline-CoC₂O₄ nano-composite, to measure the change of conductivity in nano-composite before and after Laccase immobilization and to investigate the direct electrochemistry of redox protein molecules entrapped in the matrix. Its catalytic effect on oxygen reduction reaction and performance as electrochemical sensor for oxygen detection were evaluated subsequently. Results from tests indicated this Laccase-based electrode shuttled electrons from enzyme active site T₂ as primary electron acceptor to dioxygen molecules attached chemically in the matrix, achieving the electro-reduction of O₂ in the absence of any external mediator with its apparent electron transferring rate:0.017 s^-1. This laccase-based electrode displayed favorable catalytic effect on oxygen reduction reaction (onset potential for catalysis:460 mV vs NHE, apparent turn-over frequency for oxygen reduction reaction:2.6×10^-4 s^-1). Enzymatic oxygen electro-reduction into water should be ascribed to the key process to promote the performance of biocathode. This Laccase based electrochemical sensor had such advantages as extremely low detection limit (0.20 μmol·L^-1) to oxygen monitoring, wide linear responding range of concentration (0.4~7.5 μmol·L^-1) and high affinity towards substrate (K_M=122.4 μmol·L^-1).
- Polyaniline-CoC₂O₄ nano-composite;Laccase;Direct electrochemistry;Catalytic oxygen reduction reaction;Electrochemical sensor
1. [1]
  [1] Zheng W, Ma J Y, Guo F, et al. Bio-Med. Mater. Eng., 2014, 24(1):229-235
2. [2]
  [2] Fokina O, Eipper J, Winandy L, et al. Bioresour. Technol., 2015,175:445-453
3. [3]
  [3] Zhang L L, Bai L, Xu M, et al. Nano Energy, 2015,11:48-55
4. [4]
  [4] Pankratov D V, Zeifman Y S, Morozova O V, et al. Electroanalysis, 2013,25(5):1143-1149
5. [5]
  [5] Scherbahn V, Putze M T, Dietzel B, et al. Biosens. Bioelectron., 2014,61(21):631-638
6. [6]
  [6] Liu Y, Qu X H, Guo H W, et al. Biosens. Bioelectron., 2006, 21(12):2195-2201
7. [7]
  [7] Rasmussen M, Ritzmann R E, Lee I, et al. J. Am. Chem. Soc., 2012,134(3):1458-1460
8. [8]
  [8] Ivanov I, Vidakovic-koch T, Sundmacher K J. Electroanal. Chem., 2013,690(2):68-73
9. [9]
  [9] Zelechowska K, Stolarczyk K, Lyp D, et al. Biocyber. Biomed. Eng., 2013,33(4):235-245
10. [10]
  [10] Cardoso F P, Neto S A, Fenga P G, et al. Electrochim. Acta, 2013,90(5):90-94
11. [11]
  [11] Farneth W E, Diner B A, Gierke T D, et al. Electroanal. Chem., 2005,581(2):190-196
12. [12]
  [12] Zhang L, Zhang Q, Lu X B, et al. Biosens. Bioelectron., 2007,23(1):102-106
13. [13]
  [13] Lu X B, Wen Z H, Li J H. Biomaterials, 2006,27(33):5740-5747
14. [14]
  [14] Royo B, Sosna M, Asensio A C, et al. J. Electroanal. Chem., 2013,704(9):67-74
15. [15]
  [15] Filip J, Tkac J. Bioelectrochemistry, 2014,96(2):14-20
16. [16]
  [16] Manesh K M, Santhosh P, Gopalan A I, et al. Electroanalysis, 2006,18(16):1564-1571
17. [17]
  [17] Santhosh P, Manesh K M, Lee K P, et al. Electroanalysis, 2006,18(9):894-903
18. [18]
  [18] CAO Zheng-Yan(曹正艳), YANG Chun-Ming(杨春明), LI Hai-Yin(李海银), et al. Chinese J. Appl. Chem.(应用化学), 2009,26(11):1264-1268
19. [19]
  [19] Blandford C F, Heather R S, Armstrong F A. Chem. Commun., 2007,43(17):1710-1712
20. [20]
  [20] HUANG Jun(黄俊), ZHOU Ju-Ying(周菊英), XIAO Hai-Yan(肖海燕), et al. Acta Chim. Sinica(化学学报), 2005,63(14):1343-1347
21. [21]
  [21] ZENG Han(曾涵), ZHAO Shu-Xian(赵淑贤), GONG Lan-Xin(龚兰新), et al. Chinese J. Appl. Chem.(应用化学), 2013,30(4):436-443
22. [22]
  [22] Zhao H Y, Zhou H M, Zhang J X, et al. Biosens. Bioelectron., 2009,25:463-468
23. [23]
  [23] Shleev S, Christenson A, Serezhenkov V, et al. Biochem. J., 2005,385:745-754
24. [24]
  [24] Qiu H J, Xu C X, Huang X R, et al. J. Phys. Chem. C, 2009, 113(6):2521-2525
25. [25]
  [25] Zhu Y F, Kaskel S, Shi J L, et al. Chem. Mater., 2007,19:6408-6413
26. [26]
  [26] ZENG Han(曾涵), YANG Yang(杨阳), LI Xiao-Juan(李小娟), et al. Chin. J. Anal. Chem.(分析化学), 2015,43(12):1794-1800
27. [27]
  [27] Rahman M A, Noh H B, Shim Y B. Anal. Chem., 2008,80:8020-8027
28. [28]
  [28] Osman M H, Shah A A, Walsh F C. Biosens. Bioelectron., 2011,26:3087-3102
29. [29]
  [29] ZENG Han(曾涵), LIAO Ling-Wen(廖铃文), LI Ming-Fang (李明芳), et al. Acta Phys.-Chim. Sin.(物理化学学报), 2010,26(12):3217-3224
30. [30]
  [30] Mano N, Kim H H, Zhang Y C, et al. J. Am. Chem. Soc., 2002,124(22):6480-6486
31. [31]
  [31] Mao F, Mano N, Heller A. J. Am. Chem. Soc., 2003,125(16):4951-4957
32. [32]
  [32] Kano K, Ikeda T. Anal. Sci., 2000,16(10):1013-1021
33. [33]
  [33] Katz E, Heleg-Shabtai V, Willner I, et al. Angew. Chem. Int. Ed., 1998,37(23):3253-3256
34. [34]
  [34] Pita M, Shleev S, Ruzgas T, et al. Electrochem. Commun., 2006,8(5):747-753
35. [35]
  [35] Ramasamy R P, Luckarift H R, Ivnitski D M, et al. Chem. Commun., 2010,46(33):6045-6047
36. [36]
  [36] Zheng W, Zhou H M, Zheng Y F, et al. Chem. Phys. Lett., 2008,457(4/5/6):381-385
37. [37]
  [37] Soukharev V, Mano N, Heller A. J. Am. Chem. Soc., 2004, 126(27):8368-8369
38. [38]
  [38] Farneth W E, DAmore M B. J. Electroanal. Chem., 2005, 581(2):197-205
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Direct Electrochemistry of Electrode Modified with Thin Film of Laccase Immobilized in Nano-Composite of Polyaniline-CoC2O4

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通讯作者: 陈斌, bchen63@163.com

Direct Electrochemistry of Electrode Modified with Thin Film of Laccase Immobilized in Nano-Composite of Polyaniline-CoC₂O₄