Citation: MAO Lan-Lan, ZHANG Li-Ming, DENG Yan, LÜ Zhuo-Xuan, HE Nong-Yue. Accurate Detection of DNA Based on the NaYF₄: Yb, Er Upconversion Nanoparticles[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(12): 2095-2101. doi: 10.11862/CJIC.2016.271 shu

Accurate Detection of DNA Based on the NaYF₄: Yb, Er Upconversion Nanoparticles

1.
1 Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, Hunan 412007 China;
2.
2 State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

Corresponding author: LÜ Zhuo-Xuan, HE Nong-Yue,
Received Date: 9 April 2016
Available Online: 24 September 2016
Fund Project:

A new method for improving the accuracy of DNA detection has been developed, which was based on the base stacking principle and the fluorescence of NaYF₄:Yb, Er UCNPs. Firstly, the NaYF₄:Yb, Er UCNPs were synthesized by thermal decomposition method and functionalized with denatured bovine serum albumin. Then, the denatured bovine serum albumin-functionalized OA/NaYF₄:Yb, Er UCNPs were conjugated with amino group-modified DNA probes to form upconversion fluorescence labeled probes and detect DNA. The results showed that the method of using the fluorescence of NaYF₄:Yb, Er UCNPs as a reference standard to quantitatively detect target DNA concentration had higher accuracy than that of only using a single FAM fluorescence intensity, and the operation and equipment errors was effectively avoid during the experiment. Moreover, this method can reach the detection limit as low as 5 nmol·L^-1 without amplification, displays a good linear relationship in wide concentration range and a high specificity, and also can effectively differenciate single-base mismatch sequences.
- NaYF₄:Yb, Er;DNA detection;reference standard;accuracy
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Accurate Detection of DNA Based on the NaYF4: Yb, Er Upconversion Nanoparticles

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通讯作者: 陈斌, bchen63@163.com

Accurate Detection of DNA Based on the NaYF₄: Yb, Er Upconversion Nanoparticles