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Citation: BAI Wen-Jing, LI Yun, CAO Da-Li, WU Yan-Fei, LIANG Yun-Xiao. Immobilization of Pseudomonas Fluorescens Lipase on PDA/SiO2 Macroporous Composite[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(11): 1973-1980. doi: 10.11862/CJIC.2016.261 shu

Immobilization of Pseudomonas Fluorescens Lipase on PDA/SiO2 Macroporous Composite

  • 1.

    State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China

  • Corresponding author: LIANG Yun-Xiao, 
  • Received Date: 19 May 2016
    Available Online: 7 October 2016

    Fund Project:

  • A large-sized macroporous SiO2 was prepared via a polymer template with three-dimensional (3D) skeletal structure and it was surface functionalized with polydopamine (PDA) by controlling the in situ polymerization of dopamine in its micro-channels to obtain the macroporous composite polydopamine/SiO2 (PDA/SiO2). Samples were characterized by SEM, EDX, MIP, BET, TG-DTA and FTIR. PDA/SiO2 was used to immobilize pseudomonas fluorescens lipase (PFL), immobilization conditions were optimized, and the properties of immobilized and free PFL were studied. The results show that the macroporous SiO2 has 3D continuous pass-through pore structure, the pore sizes are in the range of 300~500 nm. After modification with PDA, the pore walls of the resulting PDA/SiO2 are composed of PDA and SiO2 composite nano-film. The best activity recovery of immobilized lipase is 246% under conditions of immobilizing time of 14 h, pH of 8 and initial PFL concentration 0.4 mg·mL-1. Compared with free PFL, the optimum working temperature and pH ranges of immobilized PFL are both widened, and the thermal stability of immobilized PFL is improved significantly. Immobilized PFL has good operational and storage stabilities. The Km of immobilized PFL is lower than that of free PFL, suggesting a better affinity capacity between PFL and substrate.
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