Advanced Search

Citation: BAI Wen-Jing, LI Yun, CAO Da-Li, WU Yan-Fei, LIANG Yun-Xiao. Immobilization of Pseudomonas Fluorescens Lipase on PDA/SiO2 Macroporous Composite[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(11): 1973-1980. doi: 10.11862/CJIC.2016.261 shu

Immobilization of Pseudomonas Fluorescens Lipase on PDA/SiO2 Macroporous Composite

  • 1.

    State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China

  • Corresponding author: LIANG Yun-Xiao, 
  • Received Date: 19 May 2016
    Available Online: 7 October 2016

    Fund Project:

  • A large-sized macroporous SiO2 was prepared via a polymer template with three-dimensional (3D) skeletal structure and it was surface functionalized with polydopamine (PDA) by controlling the in situ polymerization of dopamine in its micro-channels to obtain the macroporous composite polydopamine/SiO2 (PDA/SiO2). Samples were characterized by SEM, EDX, MIP, BET, TG-DTA and FTIR. PDA/SiO2 was used to immobilize pseudomonas fluorescens lipase (PFL), immobilization conditions were optimized, and the properties of immobilized and free PFL were studied. The results show that the macroporous SiO2 has 3D continuous pass-through pore structure, the pore sizes are in the range of 300~500 nm. After modification with PDA, the pore walls of the resulting PDA/SiO2 are composed of PDA and SiO2 composite nano-film. The best activity recovery of immobilized lipase is 246% under conditions of immobilizing time of 14 h, pH of 8 and initial PFL concentration 0.4 mg·mL-1. Compared with free PFL, the optimum working temperature and pH ranges of immobilized PFL are both widened, and the thermal stability of immobilized PFL is improved significantly. Immobilized PFL has good operational and storage stabilities. The Km of immobilized PFL is lower than that of free PFL, suggesting a better affinity capacity between PFL and substrate.
  • 加载中
    1. [1]

      [1] Jaeger K E, Eggert T. Curr. Opin. Biotechnol., 2002,13:390-397

    2. [2]

      [2] Fernandez-Lafuente R. J. Mol. Catal. B:Enzym., 2010,62: 197-212

    3. [3]

      [3] Maciejewski M, Pótorak K, Kaminska J E. J. Mol. Catal. B: Enzym., 2010,62(3/4):248-256

    4. [4]

      [4] Osborn H T, Akoh C C. J. Food Sci., 2002,67(7):2480-2485

    5. [5]

      [5] Athawale V, Manjrekar N, Athawale M. J. Mol. Catal. B: Enzym., 2001,16:169-173

    6. [6]

      [6] Lima L N, Oliveira G C, Rojas M J, et al. J. Ind. Microbiol. Biotechnol., 2015,42:523-535

    7. [7]

      [7] Hasan F, Shah A A, Hameed A. Enzyme Microb. Technol., 2006,39:235-251

    8. [8]

      [8] Mateo C, Palomo J M, Fernandez-Lorente G, et al. Enzyme Microb. Technol., 2007,40:1451-1463

    9. [9]

      [9] WANG Lei(王磊), CHEN Cheng(陈诚), TIAN Mei-Juan (田美娟), et al. Chinese J. Inorg. Chem. (无机化学学报), 2013,29(4):677-688

    10. [10]

      [10] Abdallah N H, Schlumpberger M, Gaffney D A, et al. J. Mol. Catal. B:Enzym., 2014,108:82-88

    11. [11]

      [11] Monsan P. J. Mol. Catal., 1977,3(78):371-384

    12. [12]

      [12] Hudson S, Cooney J, Magner E. Angew. Chem. Int. Ed., 2008, 47:8582-8594

    13. [13]

      [13] YANG Hong-Bin(杨洪斌), CHEN Qi(陈奇), SONG Li (宋鹂), et al. Chinese J. Inorg. Chem.(无机化学学报), 2007,23(1):164-168

    14. [14]

      [14] Li Y, Gao F, Wei W, et al. J. Mol. Catal. B:Enzym., 2010, 66:182-189

    15. [15]

      [15] Jiang Y J, Wang Y P, Wang H, et al. New J. Chem., 2015, 39:978-984

    16. [16]

      [16] LI Yun(李云), LIU Xiao-Zhen(刘晓贞), LIANG Yun-Xiao (梁云霄). Acta Materiae Compositae Sinica (复合材料学报), 2016,33(3):634-642

    17. [17]

      [17] Lee H, Scherer N F, Messersmith P B. Proc. Natl. Acad. Sci. USA, 2006,103:12999-13003

    18. [18]

      [18] Lee H, Dellatore S M, Miller W M, et al. Science, 2007, 318:426-430

    19. [19]

      [19] Chao C, Zhang B, Zhai R, et al. ACS Sustainable Chem. Eng., 2014,2:396-403

    20. [20]

      [20] Ren Y H, Rivera J G, He L H, et al. BMC Biotechnol., 2011, 11:1472-6750

    21. [21]

      [21] Kilcawley K N, Wilkinson M G, Fox P F. Enzyme Microb. Technol., 2002,31:310-320

    22. [22]

      [22] Bradford M M. Ana1. Biochem., 1976,72:248-254

    23. [23]

      [23] Abdullah A Z, Sulaiman N S, Kamaruddin A H. Biochem. Eng. J., 2009,44:263-270

    24. [24]

      [24] Safari M, Ghiaci M, Jafari-Asl M, et al. Appl. Surf. Sci., 2015,342:26-33

    25. [25]

      [25] Netto C G C M, Andrade L H, Toma H E. Tetrahedron: Asymmetry, 2009,20:2299-2304

    26. [26]

      [26] Liu J, Guo H T, Zhou Q B, et al. J. Mol. Catal. B:Enzym., 2013,96:96-102

    27. [27]

      [27] TAO Wei-Hong(陶维红), YANG Li-Rong(杨立荣), XU Gang(徐刚), et al. Chem. Ind. Eng. Prog.(化工进展), 2011, 30(7):1584-1590

    28. [28]

      [28] Lee H, Rho J, Messersmith P B. Adv. Mater., 2009,21(4): 431-434

    29. [29]

      [29] ZHANG Qun(张群), ZHANG Yu-Qi(张育淇), LIU Xiao-Zhen(刘晓贞), et al. Chinese J. Inorg. Chem.(无机化学学报), 2013,23(10):2065-2070

    30. [30]

      [30] Lei L, Bai Y X, Li Y F, et al. J. Magn. Magn. Mater., 2009, 321:252-258

    31. [31]

      [31] Park K M, Kwon C W, Choi S J, et al. J. Agric. Food Chem., 2013,61:94219427

    32. [32]

      [32] Lei L, Liu X, Li Y F, et al. Mater. Chem. Phys., 2011,125: 866-871

    33. [33]

      [33] Kanimozhi S, Perinbam K. Mater. Res. Bull., 2013,48:1830-1836

  • 加载中
    1. [1]

      Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351

    2. [2]

      Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364

    3. [3]

      Yunhao Zhang ,  Yinuo Wang ,  Siran Wang ,  Dazhen Xu . Progress in Selective Construction of Functional Aromatics from Nitrogenous Cycloalkanes. University Chemistry, 2024, 39(11): 136-145. doi: 10.3866/PKU.DXHX202401083

    4. [4]

      Wenjie SHI , Fan LU , Mengwei CHEN , Jin WANG , Yingfeng HAN . Synthesis and host-guest properties of imidazolium-functionalized zirconium metal-organic cage. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 105-113. doi: 10.11862/CJIC.20240360

    5. [5]

      Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194

    6. [6]

      Yue Wu ,  Jun Li ,  Bo Zhang ,  Yan Yang ,  Haibo Li ,  Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028

    7. [7]

      Fan Wu ,  Wenchang Tian ,  Jin Liu ,  Qiuting Zhang ,  YanHui Zhong ,  Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031

    8. [8]

      Guangming YIN , Huaiyao WANG , Jianhua ZHENG , Xinyue DONG , Jian LI , Yi'nan SUN , Yiming GAO , Bingbing WANG . Preparation and photocatalytic degradation performance of Ag/protonated g-C3N4 nanorod materials. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1491-1500. doi: 10.11862/CJIC.20240086

    9. [9]

      Fugui XI , Du LI , Zhourui YAN , Hui WANG , Junyu XIANG , Zhiyun DONG . Functionalized zirconium metal-organic frameworks for the removal of tetracycline from water. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 683-694. doi: 10.11862/CJIC.20240291

    10. [10]

      Hongsheng Tang ,  Yonghe Zhang ,  Dexiang Wang ,  Xiaohui Ning ,  Tianlong Zhang ,  Yan Li ,  Hua Li . A Wonderful Journey through the Kingdom of Hazardous Chemicals. University Chemistry, 2024, 39(9): 196-202. doi: 10.12461/PKU.DXHX202403098

    11. [11]

      Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414

    12. [12]

      Yinyin Qian ,  Rui Xu . Utilizing VESTA Software in the Context of Material Chemistry: Analyzing Twin Crystal Nanostructures in Indium Antimonide. University Chemistry, 2024, 39(3): 103-107. doi: 10.3866/PKU.DXHX202307051

    13. [13]

      Shiyan Cheng ,  Yonghong Ruan ,  Lei Gong ,  Yumei Lin . Research Advances in Friedel-Crafts Alkylation Reaction. University Chemistry, 2024, 39(10): 408-415. doi: 10.12461/PKU.DXHX202403024

    14. [14]

      Yifeng TAN , Ping CAO , Kai MA , Jingtong LI , Yuheng WANG . Synthesis of pentaerythritol tetra(2-ethylthylhexoate) catalyzed by h-MoO3/SiO2. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2155-2162. doi: 10.11862/CJIC.20240147

    15. [15]

      Guang-Xu Duan , Queting Chen , Rui-Rui Shao , Hui-Huang Sun , Tong Yuan , Dong-Hao Zhang . Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO2 nano-membrane for enhancing catalytic performance. Chinese Chemical Letters, 2025, 36(2): 109751-. doi: 10.1016/j.cclet.2024.109751

    16. [16]

      . . Chinese Journal of Inorganic Chemistry, 2024, 40(11): 0-0.

    17. [17]

      Lina Guo ,  Ruizhe Li ,  Chuang Sun ,  Xiaoli Luo ,  Yiqiu Shi ,  Hong Yuan ,  Shuxin Ouyang ,  Tierui Zhang . 层状双金属氢氧化物的层间阴离子对衍生的Ni-Al2O3催化剂光热催化CO2甲烷化反应的影响. Acta Physico-Chimica Sinica, 2025, 41(1): 2309002-. doi: 10.3866/PKU.WHXB202309002

    18. [18]

      Xinpin Pan , Yongjian Cui , Zhe Wang , Bowen Li , Hailong Wang , Jian Hao , Feng Li , Jing Li . Robust chemo-mechanical stability of additives-free SiO2 anode realized by honeycomb nanolattice for high performance Li-ion batteries. Chinese Chemical Letters, 2024, 35(10): 109567-. doi: 10.1016/j.cclet.2024.109567

    19. [19]

      Wenyan Dan ,  Weijie Li ,  Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060

    20. [20]

      Yanxin Wang ,  Hongjuan Wang ,  Yuren Shi ,  Yunxia Yang . Application of Python for Visualizing in Structural Chemistry Teaching. University Chemistry, 2024, 39(3): 108-117. doi: 10.3866/PKU.DXHX202306005

Get Citation
PDF
XML
Metrics
  • PDF Downloads(0)
  • Abstract views(375)
  • HTML views(50)

通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888
Powered By info@rhhz.net

/

DownLoad:  Full-Size Img  PowerPoint
Return