Citation: ZENG Han, YANG Yang, ZHAO Shu-Xian. Catalytic Effect of Two Kinds of Functionalized Nano-Gold Particles with Immobilized Enzymes Modified Electrodes[J]. Chinese Journal of Inorganic Chemistry, ;2015, (12): 2305-2314. doi: 10.11862/CJIC.2015.316 shu

Catalytic Effect of Two Kinds of Functionalized Nano-Gold Particles with Immobilized Enzymes Modified Electrodes

1.
College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumuqi 830054, China

Corresponding author: ZENG Han,
Received Date: 30 August 2015
Available Online: 20 October 2015
Fund Project: 国家自然科学基金(No.21363024) (No.21363024)新疆师范大学博士科研启动基金(No.XJNUBS1228) (No.XJNUBS1228)新疆维吾尔自治区2013年度高校科研计划青年教师培育项目(No.XJEDU2013S29) (No.XJEDU2013S29)新疆师范大学研究生科技创新项目(No.XSY201502009)资助。 (No.XSY201502009)

Synthesized 4-mercaptobenzoic acid functionalized nano-gold particles and poly(vinylpyridine) overlapped nanogold-particles, were used as enzyme carriers to prepare two kinds of novel enzyme-based electrodes, respectively. Two prototypes of enzyme-based fuel cells were fabricated on the basis of previously described electrodes. Morphology of matrix with immobilized enzymes, influences of interaction between enzyme molecules and carriers on spectrometric characteristics of electrode surface anchored enzyme molecules, direct electron transfer dynamics between enzyme active centers and electrodes and catalytic function in substrate involved reaction, were investigated by the means of electrochemical method together with such techniques as ultra-violet/visible spectrometry(UV-Vis) and transmission electron microscope(TEM). Energy out-put performances for two kinds of fabricated enzyme-based fuel cells were evaluated and compared systematically. Results from test indicated 4-mercaptobenzoic acid surface-tailored nano-gold particles with enzymes modified electrodes displayed direct electron transfer between enzyme active sites and electrode, revealing favorable catalytic effect on glucose electro-oxidation and oxygen electro-reduction(catalytic reaction onset potential for glucose oxidationn and oxygen reduction: -0.03 and 0.96 V, turn-over frequency of substrates: 1.3 and 0.5 s^-1, respectively). Reproducibility, long-term usability, acid/base endurance and thermal stability in catalytic function of previously mentioned electrode were preferable. Catalytic effect in substrate-related reaction of enzyme-based electrode increased with the thickness of self-assembled immobilized enzyme layers until approaching to maximal catalytic current. Test on performance of fuel cell showed open circuit voltage(OCV, 0.88 V), maximal out-put energy density(864.0 μW·cm^-2) and excellent long-term stability(retaining above 80% of optimal energy out-put after storage for 3 weeks).
- laccase;glucose oxidase;functionalized nano-gold particle;direct electron transfer;catalytic function;enzymatic biofuel cell
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