Citation: WEN Chang-Chun, LEI Wen-Qi, SHEN Xing-Can, JI Shi-Chen, JIANG Bang-Ping, LIANG Hong. Comparative Interaction Mechanisms Between Cells and Gold Nanoparticles Modified with Different Chemical Functional Groups[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(9): 1903-1912. doi: 10.11862/CJIC.2015.253 shu

Comparative Interaction Mechanisms Between Cells and Gold Nanoparticles Modified with Different Chemical Functional Groups

1.
Key Laboratory of Medicinal Chemical Resources and Molecular Engineering, Ministry of Education, Guangxi Normal University, Guilin 541004, China

Corresponding author: SHEN Xing-Can, LIANG Hong,
Received Date: 18 June 2015
Available Online: 4 August 2015
Fund Project: 国家自然科学基金(No.21161003,21364002) (No.21161003,21364002)广西自然科学基金杰青(2013GXNSFGA019001) (2013GXNSFGA019001)教育部新世纪优秀人才支持计划(NCET-13-0743) (NCET-13-0743)药用资源化学与药物分子工程教育部重点实验室主任基金(2015-A)资助项目。 (2015-A)

Chemical functional groups of -CH₃, -COOH and -OH have been introduced to the surface of AuNPs, separately. The AuNPs, Au-OH NPs, Au-COOH NPs and Au-CH₃ NPs are spherical with dimension of (15.6±3.2) nm, displaying negative ζ potentials. The cytotoxicity of these AuNPs has been evaluated by methylthiazoletetrazolium (MTT) assay against Hela cells and MCG-803 cells in vitro, separately. MTT data reveal that the surface unmodified AuNPs exhibit low cytotoxicity at the highest concentration of 197 ng·mL^-1 for both HeLa and MCG-803 cells in vitro. The surface modified AuNPs can further decrease the inherently cytotoxicity that follows the order AuNPs > Au-CH₃ NPs > Au-COOH NPs≈Au-OH NPs. Cell cycle analysis indicates that AuNPs cause cell cycle slightly arrest at the G₂/M phase. Micro-Raman spectra of individual living HeLa cells demonstrate that the backbone and nucleic bases of DNA as well as the polar headgroup of phospholipid in cells are the probable target binding sites of AuNPs and Au-CH₃ NPs. Whereas, the interfacial interactions are significantly reduced when cells are treated with Au-COOH NPs and Au-OH NPs. Our results on the interaction mechanisms between AuNPs and cells demonstrate that AuNPs modified with surface functional groups of -COOH or -OH can improve their cytocompatibility.
- gold nanoparticles (AuNPs);surface functional groups;cycotoxicity;molecular mechanisms
1. [1]
  [1] Yeh Y C, Creran B, Rotello V M. Nanoscale, 2012,4:1871-1880
2. [2]
  [2] Daniel M C, Astruc D. Chem. Rev., 2004,104:293-346
3. [3]
  [3] Turkevich J, Stevenson P C, Hillier J. Discuss. Faraday Soc., 1951,11:55-75
4. [4]
  [4] Frens G. Nat. Phys. Sci., 1973,241:20-22
5. [5]
  [5] Giljohann D A, Seferos, D S, Daniel W L, et al. Angew. Chem. Int. Ed., 2010,49:3280-3294
6. [6]
  [6] Wang Z, Tan B, Hussain I, et al. Langmuir, 2007,23:885-895
7. [7]
  [7] Ding Y, Bian X, Yao W, et al. ACS Appl. Mater. Inter., 2010,2:1456-1465
8. [8]
  [8] Huang P, Bao L, Zhang C, et al. Biomaterials, 2011,32:9796-9809
9. [9]
  [9] Wu P, Hwang K, Lan T, et al. J. Am. Chem. Soc., 2013,135:5254-5257
10. [10]
  [10] Bartczak D, Nitti S, Millar T M, et al. Nanoscale, 2012,4:4470-4472
11. [11]
  [11] Park J, Park J H, Ock K S, et al. J. Colloid Interface Sci., 2011,363:105-113
12. [12]
  [12] Ghosh R, Singh L C, Shohet J M, et al. Biomaterials, 2013,34:807-816
13. [13]
  [13] Wang F, Wang Y-C, Dou S, et al. ACS Nano, 2011,5:3679-3692
14. [14]
  [14] Heo D N, Yang D H, Kwon K. Biomaterials, 2012,33:856-866
15. [15]
  [15] Mieszawska A J, Mulder W J M, Fayad Z A, et al. Mol. Pharmaceutics, 2013,10:831-847
16. [16]
  [16] Jans H, Huo Q. Chem. Soc. Rev., 2012,41:2849-2866
17. [17]
  [17] Pan Y, Neuss S, Leifert A, et al. Small, 2007,3:1941-1949
18. [18]
  [18] Goodman C M, McCusker C D, Yilmaz T, et al. Bioconjugate Chem., 2004,15:897-900
19. [19]
  [19] Connor E E, Mwamuka J, Gole A, et al. Small, 2005,1:325-327
20. [20]
  [20] Pan Y, Leifert A, Ruau D, et al. Small, 2009,5:2067-2076
21. [21]
  [21] Avvakumova S, Scari G, Porta F. RSC Adv., 2012,2:3658-3661
22. [22]
  [22] Park J-W, Shumaker-Parry J S. ACS Nano, 2015,9:1665-1682
23. [23]
  [23] Zhou Y, Wang S X, Zhang K, et al. Angew. Chem., 2008, 120:7564-7566
24. [24]
  [24] Zhao W, Chiuman W, Lam J C F, et al. J. Am. Chem. Soc., 2008,130:3610-3618
25. [25]
  [25] Nuzzo R G, Zegarski B R, Dubois L H. J. Am. Chem. Soc., 1987,109:733-740
26. [26]
  [26] Chen F, Li X, Hihath J, et al. J. Am. Chem. Soc., 2006,128:15874-15881
27. [27]
  [27] Jiang B P, Zhang L, Zhu Y, et al. J. Mater. Chem. B, 2015,3:3767-3776
28. [28]
  [28] Jeyaraj M, Arun R, Sathishkumar G, et al. Mater. Res. Bull., 2014,52:15-24
29. [29]
  [29] Puppels G J, de Mul F F, Otto C, et al. Nature, 1990,347:301-303
30. [30]
  [30] Konorov S O, Schulze H G, Piret J M, et al. J. Raman Spectrosc., 2011,42:1135-1141
31. [31]
  [31] Bush S F, Adams R G, Levin I W. Biochemistry, 1980,19:4429-4436
32. [32]
  [32] Notingher I, Verrier S, Haque S, et al. Biopolymer, 2003,72:230-240
33. [33]
  [33] Zoladek A, Pascut F C, Patel P, et al. J. Raman Spectrosc., 2011,42:251-258
34. [34]
  [34] Pyrgiotakis G, Kundakcioglu O E, Pardalos P M, et al. J. Raman Spectrosc., 2011,42:1222-1231
1. [1]
  Jing JIN , Zhuming GUO , Zhiyin XIAO , Xiujuan JIANG , Yi HE , Xiaoming LIU . Tuning the stability and cytotoxicity of fac-[Fe(CO)₃I₃]^- anion by its counter ions: From aminiums to inorganic cations. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 991-1004. doi: 10.11862/CJIC.20230458
2. [2]
  Xinyu Zhu , Meili Pang . Application of Functional Group Addition Strategy in Organic Synthesis. University Chemistry, 2024, 39(3): 218-230. doi: 10.3866/PKU.DXHX202308106
3. [3]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
4. [4]
  Danqing Wu , Jiajun Liu , Tianyu Li , Dazhen Xu , Zhiwei Miao . Research Progress on the Simultaneous Construction of C—O and C—X Bonds via 1,2-Difunctionalization of Olefins through Radical Pathways. University Chemistry, 2024, 39(11): 146-157. doi: 10.12461/PKU.DXHX202403087
5. [5]
  Huan LI , Shengyan WANG , Long Zhang , Yue CAO , Xiaohan YANG , Ziliang WANG , Wenjuan ZHU , Wenlei ZHU , Yang ZHOU . Growth mechanisms and application potentials of magic-size clusters of groups Ⅱ-Ⅵ semiconductors. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1425-1441. doi: 10.11862/CJIC.20240088
6. [6]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
7. [7]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
8. [8]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
9. [9]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
10. [10]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
11. [11]
  Zhonghua Xi , Xuanfeng Kong , Jinyue Yang , Bin Liu , Tingyu Zhu , Hui Zhang , Wenwei Zhang . Construction of Public Teaching Instrument Platform and Exploration of Opening Mechanism. University Chemistry, 2024, 39(7): 200-206. doi: 10.12461/PKU.DXHX202405123
12. [12]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
13. [13]
  Weihan Zhang , Menglu Wang , Ankang Jia , Wei Deng , Shuxing Bai . 表面硫物种对钯-硫纳米片加氢性能的影响. Acta Physico-Chimica Sinica, 2024, 40(11): 2309043-. doi: 10.3866/PKU.WHXB202309043
14. [14]
  Xuan Zhou , Yi Fan , Zhuoqi Jiang , Zhipeng Li , Guowen Yuan , Laiying Zhang , Xu Hou . Liquid Gating Mechanism and Basic Properties Characterization: a New Experimental Design for Interface and Surface Properties in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 113-120. doi: 10.12461/PKU.DXHX202407111
15. [15]
  Liang MA , Honghua ZHANG , Weilu ZHENG , Aoqi YOU , Zhiyong OUYANG , Junjiang CAO . Construction of highly ordered ZIF-8/Au nanocomposite structure arrays and application of surface-enhanced Raman spectroscopy. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1743-1754. doi: 10.11862/CJIC.20240075
16. [16]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
17. [17]
  Heng Chen , Longhui Nie , Kai Xu , Yiqiong Yang , Caihong Fang . 两步焙烧法制备大比表面积和结晶性增强超薄g-C₃N₄纳米片及其高效光催化产H₂O₂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406019-. doi: 10.3866/PKU.WHXB202406019
18. [18]
  Zian Fang , Qianqian Wen , Yidi Wang , Hongxia Ouyang , Qi Wang , Qiuping Li . The Test Paper for Metal Ion: A Popular Science Experiment Based on Color Aesthetics. University Chemistry, 2024, 39(5): 108-115. doi: 10.3866/PKU.DXHX202310032
19. [19]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
20. [20]
  Yong Shu , Xing Chen , Sai Duan , Rongzhen Liao . How to Determine the Equilibrium Bond Distance of Homonuclear Diatomic Molecules: A Case Study of H₂. University Chemistry, 2024, 39(7): 386-393. doi: 10.3866/PKU.DXHX202310102

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(289)
HTML views(42)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142