Citation: TANG Qian, SHI Shan-Shan, CAO Hong-Yu, GUO Xiang-Jin, ZHANG Tao, ZHENG Xue-Fang. Reaction Mechanism of Cytochrome C with NO[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(8): 1511-1519. doi: 10.11862/CJIC.2015.216 shu

Reaction Mechanism of Cytochrome C with NO

TANG Qian ^1,2 ,
SHI Shan-Shan ^1,2 ,
CAO Hong-Yu ^1,2 ,
GUO Xiang-Jin ² ,
ZHANG Tao ¹ ,
ZHENG Xue-Fang ^1,2,,

1.
1. College of Life Science and Biotechnology, Dalian University, Dalian, Liaoning 116622, China;
2.
2. Liaoning Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian, Liaoning 116622, China

Corresponding author: ZHENG Xue-Fang,
Received Date: 5 September 2014
Available Online: 7 May 2015
Fund Project: 国家自然科学基金 (No.21271036, 20871024) (No.21271036, 20871024)辽宁省教育厅科学技术研究 (No.L2013470, L2013471)资助项目。 (No.L2013470, L2013471)

The research of cytochrome C (Cyt C) reacting with NO donor drugs(proliNONOate) has been focused on electrochemistry and medical treatment, while the study about reacting with NO gas is ignored. The previous study usually concentrated on Q band changes, but the Soret band was hardly mentioned. In this paper, we studied the reaction of two states of Cyt C with NO gas and the dissociation process by Soret band and Q band spectra, using ultraviolet-visible absorption spectra, electron paramagnetic resonance (EPR) spectra, ultraviolet-visible time course absorption spectra and synchronous fluorescence. The spectroscopic data showed that, ferric cytochrome C(Fe(Ⅲ)-Cyt C) and ferrous cytochrome C(Fe(Ⅱ)-Cyt C) could react with NO to convert to cytochrome C coordination compound(Cyt C-NO) with different mechanisms. Fe(Ⅱ)-Cyt C reacting with NO generated Fe(Ⅲ)-Cyt C firstly, then it combined with NO, while Fe(Ⅲ)-Cyt C can combine with NO directly. However, Cyt C-NO is not a stable coordinated macromolecule. With a small amount of NO bubbled into the sample, Cyt C-NO dissociated rapidly with rate constant value of (0.005 07±0.001) s^-1, which is one-tenth of Cyt C-NO with NO donor drugs; while under excessive NO gas condition, the dissociation process was prevented. According to the experimental data, the coordination mechanism of the reaction between Cyt C and NO is that gas molecule gets access into the cavity of Heme, making the separation of Fe-S and the formation of Fe-N. Cyt C can react with NO gas directly, the Cyt C-NO is more stable than that reacted with NO donor drugs, and the Soret band have an obvious change. These results will be significant for us to remit the oxidative stress in cells and detect the respiratory enzymes change which could monitor the cell apoptosis and some diseases by NO.
- cytochrome c;nitric oxide;spectrometry;coordination reaction;dissociation
1. [1]
  [1] Wittenberg B. J. Physiol. Rev., 1970,50(4):559-636
2. [2]
  [2] Hills B A. Science, 1973,182(4114):823-825
3. [3]
  [3] Cassina A M, Hodara R, Souza J M, et al. J. Biol. Chem., 2000,275(28):21409-21415
4. [4]
  [4] Soldatova A V, Ibrahim M, Olson J S, et al. J. Am. Chem. Soc., 2010,132(13):4614-4625
5. [5]
  [5] Rong Z, Cooper C E. Adv. Exp. Med. Biol., 2013,789:361-368
6. [6]
  [6] Larson S K, Dwyer D S, Lo H H, et al. Biochem. Biophys. Res. Commun., 2006,342(3):991-995
7. [7]
  [7] Godoy L C, Munoz-Pinedo C, Castro L, et al. PNAS USA, 2009,106(8):2653-2658
8. [8]
  [8] Kanokwiroon K, Chatpun S. J. Asian Trans. Sci., 2014,8(2):100-104
9. [9]
  [9] Basu S, Keszler A, Azarova N A, et al. Free Radical Biol. Med., 2010,48(2):255-263
10. [10]
  [10] Basu S, Azarova N A, Font M D, et al. J. Biol. Chem., 2008,283(47):32590-32597
11. [11]
  [11] Jaeheung Park T L, Manho L. J. Phys. Chem. B, 2013:12039-12050
12. [12]
  [12] Herold S, Exner M, Nauser T. Biochemistry, 2001,40(11):3385-3395
13. [13]
  [13] Yukl E T, Vries S, Moënne-Loccoz P. J. Am. Chem. Soc., 2009,131(21):7234-7235
14. [14]
  [14] Gow A J, Luchsinger B P, Pawloski J R, et al. PNAS USA, 1999,96(16):9027-9032
15. [15]
  [15] Kruglik S G, Yoo B K, Franzen S, et al. PNAS USA, 2010, 107(31):13678-13683
16. [16]
  [16] Sharpe M A, Cooper C E. Biochem. J., 1998,332(1):9-19
17. [17]
  [17] Silkstone R S, Mason M G, Nicholls P, et al. Radical Bio. Med., 2012,52(1):80-87
18. [18]
  [18] Kim J, Park J, Lee T, et al. J. Phy. Chem. B, 2012,116(46):13663-13671
19. [19]
  [19] Jitkaew S, Witasp E, Zhang S, et al. J. Leukocyte Biol., 2009,85(3):427-437
20. [20]
  [20] Kagan V E, Bayir A, Bayir H, et al. Mol. Nutr. Food. Res., 2009,53(1):104-114
21. [21]
  [21] Kagan V E, Bayir H A, Belikova N A, et al. Free Radical Biol. Med., 2009,46(11):1439-1453
22. [22]
  [22] Grubina R, Basu S, Tiso M, et al. J. Biol. Chem., 2008,283(6):3628-3638
23. [23]
  [23] Margoliash E, Frohwirt N. J. Biochem., 1959,71(3):570-572
24. [24]
  [24] Zhao X J, Sampath V, Caughey W S. Biochem. Biophys. Res. Commun, 1994,204(2):537-543
25. [25]
  [25] Gray A L, Raphael A L. Proc. Struct. Funct. Genet., 1989,6(3):338-340
26. [26]
  [26] Stellwagen E, Cass R. Biochem. Biophys. Res. Commun, 1974,60(1):371-375
27. [27]
  [27] WANG Cui-Ping(王翠平), YE Liu(叶柳), XIE Jian-An (谢建安), et al. Res. Explor. Lab.(实验室研究与探索), 2013,32(5):5-7
28. [28]
  [28] Kapralov A A, Kurnikov I V, Vlasova I I, et al. Biochem., 2007,49(46):14232-14244
29. [29]
  [29] Smagghe B J, Trent J T, et al. PLoS One, 2008,3(4):1-10
30. [30]
  [30] Jayakumari N R, Reghuvaran A C, et al. Biol. Chem., 2014, 08(2):35-44
31. [31]
  [31] Orii Y, Shimada H. J. Biochem., 1978,84(6):1543-1552
32. [32]
  [32] Balakrishnan G, Hu Y, Oyerinde O F, et al. J. Am. Chem. Soc., 2007,129(3):504-505
33. [33]
  [33] JIAN Ju(剑菊), QU Xiao-Gang(曲小刚), LU Tian-Hong (陆天红), et al. Chem. J. Chinese Universities(高等学校化学学报), 1995,16(8):1270-1274
34. [34]
  [34] George T, Doreen W S, English A M, et al. Biochemistry, 1998,37(7):2004-2016
35. [35]
  [35] Takano T, Dickerson R E. J. Mol. Biol., 1981,153(1):95-115
1. [1]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
2. [2]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
3. [3]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
4. [4]
  Ming Li , Zhaoyin Li , Mengzhu Liu , Shaoxiang Luo . Unveiling the Artistry of Mordant Dyeing: The Coordination Chemistry Beneath. University Chemistry, 2024, 39(5): 258-265. doi: 10.3866/PKU.DXHX202311085
5. [5]
  Quanguo Zhai , Peng Zhang , Wenyu Yuan , Ying Wang , Shu'ni Li , Mancheng Hu , Shengli Gao . Reconstructing the “Fundamentals of Coordination Chemistry” in Inorganic Chemistry Course. University Chemistry, 2024, 39(11): 117-130. doi: 10.12461/PKU.DXHX202403065
6. [6]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
7. [7]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
8. [8]
  Yingran Liang , Fei Wang , Jiabao Sun , Hongtao Zheng , Zhenli Zhu . Construction and Application of a New Experimental Device for Determination of Alkaline Metal Elements by Plasma Atomic Emission Spectrometry Based on Solution Cathode Glow Discharge: An Alternative Approach for Fundamental Teaching Experiments in Emission Spectroscopy. University Chemistry, 2024, 39(5): 380-387. doi: 10.3866/PKU.DXHX202312024
9. [9]
  Min WANG , Dehua XIN , Yaning SHI , Wenyao ZHU , Yuanqun ZHANG , Wei ZHANG . Construction and full-spectrum catalytic performance of multilevel Ag/Bi/nitrogen vacancy g-C₃N₄/Ti₃C₂T_x Schottky junction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1123-1134. doi: 10.11862/CJIC.20230477
10. [10]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
11. [11]
  Chunyang Zheng , Shiyu Liu , Nuo Yi , Hong Shang . The Adventures in the Kingdom of Plant Pigments. University Chemistry, 2024, 39(9): 170-176. doi: 10.3866/PKU.DXHX202308085
12. [12]
  Danqing Wu , Jiajun Liu , Tianyu Li , Dazhen Xu , Zhiwei Miao . Research Progress on the Simultaneous Construction of C—O and C—X Bonds via 1,2-Difunctionalization of Olefins through Radical Pathways. University Chemistry, 2024, 39(11): 146-157. doi: 10.12461/PKU.DXHX202403087
13. [13]
  Shitao Fu , Jianming Zhang , Cancan Cao , Zhihui Wang , Chaoran Qin , Jian Zhang , Hui Xiong . Study on the Stability of Purple Cabbage Pigment. University Chemistry, 2024, 39(4): 367-372. doi: 10.3866/PKU.DXHX202401059
14. [14]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
15. [15]
  Xuyang Wang , Jiapei Zhang , Lirui Zhao , Xiaowen Xu , Guizheng Zou , Bin Zhang . Theoretical Study on the Structure and Stability of Copper-Ammonia Coordination Ions. University Chemistry, 2024, 39(3): 384-389. doi: 10.3866/PKU.DXHX202309065
16. [16]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
17. [17]
  Shipeng WANG , Shangyu XIE , Luxian LIANG , Xuehong WANG , Jie WEI , Deqiang WANG . Piezoelectric effect of Mn, Bi co-doped sodium niobate for promoting cell proliferation and bacteriostasis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1919-1931. doi: 10.11862/CJIC.20240094
18. [18]
  Peng GENG , Guangcan XIANG , Wen ZHANG , Haichuang LAN , Shuzhang XIAO . Hollow copper sulfide loaded protoporphyrin for photothermal-sonodynamic therapy of cancer cells. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1903-1910. doi: 10.11862/CJIC.20240155
19. [19]
  Gonglan Ye , Xia Yin , Feng Xu , Peng Yang , Yingpeng Wu , Huilong Fei . Innovations in “Four-in-One” Inorganic Chemistry Education. University Chemistry, 2024, 39(8): 136-141. doi: 10.3866/PKU.DXHX202401071
20. [20]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(561)
HTML views(148)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142