Citation: TIAN Shu-Fang, ZOU Xue-Yan, LU Hai-Tao, HE Jian-Ying, CHEN Dan-Yun, ZHAO Yan-Bao, GUO Jing-Yu. Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(7): 1329-1334. doi: 10.11862/CJIC.2015.191 shu

Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein

  • Corresponding author: ZOU Xue-Yan, 
  • Received Date: 24 January 2015
    Available Online: 25 April 2015

    Fund Project: 河南省教育厅科学技术重点研究项目(No.14B150003) (No.14B150003) 国家自然科学基金(No.21271062)资助项目。 (No.21271062)

  • Hollow silica nanospheres with thiol groups (SiO2-SH NSs) have been successfully prepared through a hydrothermal route. Then the SiO2-SH NSs was further modified by conjugating iminodiacetic acid (IDA) to bear dual chelating groups (-SH and -COOH). After chelating Ni2+, these hollow NSs with dual chelating groups were applied to purify histidine-tagged (His-tagged) proteins. Results show that these hollow NSs can be widely used to separate His-tagged proteins and have a good reused ability.
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