Citation: TIAN Shu-Fang, ZOU Xue-Yan, LU Hai-Tao, HE Jian-Ying, CHEN Dan-Yun, ZHAO Yan-Bao, GUO Jing-Yu. Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(7): 1329-1334. doi: 10.11862/CJIC.2015.191 shu

Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein

1.
1 College of Chemistry and Chemical Enginerring, Henan University, Kaifeng, Henan 475004, China;
2.
2 Key Laboratory for Special Functional Materials, Henan University, Kaifeng, Henan 475004, China;
3.
3 Henan University Minsheng College, Kaifeng, Henan 475004, China

Corresponding author: ZOU Xue-Yan,
Received Date: 24 January 2015
Available Online: 25 April 2015
Fund Project: 河南省教育厅科学技术重点研究项目(No.14B150003) (No.14B150003) 国家自然科学基金(No.21271062)资助项目。 (No.21271062)

Hollow silica nanospheres with thiol groups (SiO₂-SH NSs) have been successfully prepared through a hydrothermal route. Then the SiO₂-SH NSs was further modified by conjugating iminodiacetic acid (IDA) to bear dual chelating groups (-SH and -COOH). After chelating Ni²⁺, these hollow NSs with dual chelating groups were applied to purify histidine-tagged (His-tagged) proteins. Results show that these hollow NSs can be widely used to separate His-tagged proteins and have a good reused ability.
- hollow microspheres;silica;separation of protein;functionalization;affinity
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