Citation:
Huai Na LI, Yun Xiang CI. A NEW RAPID FLUOROMETRIC METHOD FOR DETERMINATION OF SULFONAMIDES[J]. Chinese Chemical Letters,
;1995, 6(6): 495-496.
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A new fluorometric method for the determination of sulfonamides is described, based on the formation of a fluorescent inclusion complex of carbonic anhydrase (CA) with dansylamide (DNSA).The fluorescence maximum emission of the complex is at 460nm with excitation at 280nm.Dissociation constants were determined for the CA-sulfonamide complexes.Limits of detection for DNSA, sulfanilamide(SAN) and ptoluenesulfonamide (PTSN) were 0.84, 19.5 and 6.1ng/ml, respectively.The recommended method was applied to the determination of sulfonamides in cow milk at ng/ml level.
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