Citation: Yan YAN, Rui Xian LIU, Yin Mao WEI, Ye Hua SHEN, Xin Du GENG. Isoentropic and Isoenthalpic Temperatures of Protein Unfolding in Hydrophobic Interaction Chromatography[J]. Chinese Chemical Letters, ;2006, 17(1): 105-108. shu

Isoentropic and Isoenthalpic Temperatures of Protein Unfolding in Hydrophobic Interaction Chromatography

1.
Institute of Modern Separation Science, Northwest University, Xi'an 710069;
2.
Contemporary Educational Technology Center, Northwest University, Xi'an 710069

Received Date: 20 April 2005

The thermal behaviors of five proteins in hydrophobic interaction chromatography (HIC) were investigated in the temperature range from 0 to 50℃. The thermodynamic parameters (△H°, △S°, △C_p°and △G°) of these proteins in the process of retention and unfolding were determined.The existence of enthalpy and entropy convergence with temperature was confirmed. The differences of the isoentropic and isoenthalpic temperatures for protein unfolding in HIC system from the traditional solution were elucidated.
- Column liquid chromatography,
- hydrophobic interaction chromatography,
- protein unfolding,
- thermodynamic convergence
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