Citation: Xiang Qin LIN, Xiao Hua JIANG, Li Ping LU. DNA Nano-netting Intertexture on Carbon Electrodes[J]. Chinese Chemical Letters, ;2004, 15(8): 997-1000. shu

DNA Nano-netting Intertexture on Carbon Electrodes

Department of Chemistry, University of Science and Technology of China, Hefei 230026

Received Date: 14 July 2003

Native calf thymus double stranded DNA (ct-dsDNA) is successfully immobilized from solution onto carbon substrates by covalent linkages under an optimized deposition potential of 1.8±0.3 V vs.50 mmol/L NaCl-Ag/AgCl.The long chain DNA fabricates a layer of well conductive nano-netting intertexture, which is stable in pH 14 alkaline solution and in boiling water.The ct-dsDNA modified carbon fiber disk electrode shows two to three orders of magnitude enlarged electrode effective surface area and similarly enlarged voltammetric responses to Co(phen)₃³⁺ and dopamine.Thermal dissociated single stranded ct-DNA can also lead to similar result.This modified electrode will find wide applications in the fields of DNA-based electrochemical biosensors.
- Calf thymus DNA,
- immobilization,
- carbon fiber electrode,
- electrochemical biosensor
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