Citation: Jian Quan LU, Wei Wei HE, Xing Wang ZHOU. Chemiluminescence of Luminol-potassium Ferricyanide with Benserazide and Application in Analytical Chemistry[J]. Chinese Chemical Letters, ;2006, 17(9): 1233-1235. shu

Chemiluminescence of Luminol-potassium Ferricyanide with Benserazide and Application in Analytical Chemistry

1.
Hubei Key Laboratory of Bioanalytical Technology, Hubei Normal University, Huangshi 435002;
2.
Department of Chemistry, Central China Normal University, Wuhan 430079

Received Date: 3 April 2006

Fund Project: This work is partially supported by the Science Foundation of the National Education Department (No. 206096), the Education Department of Hubei Province (No. Z200522002) and the Open Foundation of Hubei Key Laboratory of Biotechnology in Traditional Chinese medicine (No. 20040201).

The enhancing effect of benserazide on the chemiluminescence of luminol-potassium ferricyanide in alkaline medium was investigated, and a sensitive method for detecting benserazide has been developed by flow-injection chemiluminescence's. The experimental results showed that the emission intensity is proportional to the concentration of benserazide in the range of 0.01-1.0 μg/mL. The detection limit (S/N=3) for benserazide turned out to be 0.9 ng/mL, and the relative standard deviation for 11 determinations of a 0.10 μg/mL benserazide was 2.4%. The method was satisfactorily applied to the determination of benserazide in spiked urine samples.
- Chemiluminescence,
- luminol,
- benserazide,
- detection
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