Citation: Xin XIE, Xu ZHANG, Bing Bin YU, Wei Yang FEI. Preparation and Characterization of Super-paramagnetic Nano-beads for DNA Isolation[J]. Chinese Chemical Letters, ;2004, 15(5): 597-600. shu

Preparation and Characterization of Super-paramagnetic Nano-beads for DNA Isolation

1.
State Key Laboratory of Extraction Separation Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084;
2.
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084;
3.
Faculty of Science, Sichuan Agricultural University, Sichuan 625014

Received Date: 6 May 2003

Fund Project: This work was supported by the project of National Outstanding Youth Science Foundation of China (39825108) and the Key Project of National Natural Science Foundation of China (39889001).

Unique coupling reagent, bis-(2-hydroxyethyl methacrylate) phosphate was used to prepare coated and functionalized superparamagnetic nanobeads, leading to a simple, effective method for coating the nanobeads. With this method, the thickness of the coating layer and the functional group contents on the nano-beads could be controlled by changing the quantity of the coated monomers. The nanobeads were characterized by means of transmission electron microscopy (TEM) and Fourier transformation infrared spectroscopy (FTIR). The carboxyl-modified magnetic nano-beads were employed to streamline the protocol of isolation of genomic DNA from the human whole blood.
- Coating,
- superparamagnetic nano-beads,
- coupling reagent,
- DNA isolation
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