Citation: Rui BI, Pu Dun ZHANG, Chao Qing DONG, Ji Cun REN. Combination of Micro-fluidic Chip with Fluorescence Correlation Spectroscopy for Single Molecule Detection[J]. Chinese Chemical Letters, ;2006, 17(4): 521-524. shu

Combination of Micro-fluidic Chip with Fluorescence Correlation Spectroscopy for Single Molecule Detection

College of Chemistry & Chemical Engineering, Shanghai Jiaotong University, Shanghai 200240

Received Date: 8 October 2005

Fund Project: This work was financially supported by the National Natural Science Foundation of China. (No.20271033, 20335020, 90408014).

A single molecule detection technique was developed by the combination of a single channel poly (dimethylsiloxane)/glass micro-fluidic chip and fluorescence correlation spectroscopy (FCS). This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
- Fluorescence correlation spectroscopy,
- micro-fluidic chip,
- single molecule detection
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