Citation: Gui Lin WEI, Zhen Hua SHAN, Ming Chen PAN, Zhi Hong GAO. Removal of Endotoxin from Human Serum Albumin Solutions by Hydrophobic and Cationic Charged Membrane[J]. Chinese Chemical Letters, ;2000, 11(4): 357-360. shu

Removal of Endotoxin from Human Serum Albumin Solutions by Hydrophobic and Cationic Charged Membrane

a Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012;
b Dalian Medical University 1st Teaching Hospital

Received Date: 22 July 1999
Revised Date: 28 December 1999

Fund Project: This work was supported by the National Natural Science Foundation of China.

A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the cationic charged groups as affinity ligands. The prepared membrane can be used for the removal of endotoxin from human serum albumin (HSA) solutions. With a cartridge of 20 sheets affinity membrane of 47 mm diameter, the endotoxin level in HSA solution can be reduced to 0.027 eu/mL. Recovery of HSA was over 95%.
- Hydrophobic and cationic charged membrane,
- endotoxin removal
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