Citation: Li SHEN, Bo Hui XIONG, Run Zhi CONG, Jun De WANG. Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography[J]. Chinese Chemical Letters, ;1999, 10(7): 583-586. shu

Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography

Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012

Received Date: 1 January 1999

Fund Project: This work is supported by the National Natural Science Fundation of China.

Rigid PSDVB microbeads have been modified with poly（vinyl alcohol) (PVA) adsorbed on their surface to produce an affinny medium. Then Cibacron Blue F3GA was covalentlv attached to the supports. The initial concentration of PVA has effect on the adsorption of PVA. The non-specific interaction of bovine serum albumin (BSA) on the microbeads decreases、、ith the PVA adsorbed. The pH stability test shows that the aftinitv medium is stable up to pH 11.0. And it has specific interaction with lysoryme, but not with pepsin.
- PSDVB microbeads,
- PVA,
- affinity medium,
- Cibacron Blue F3GA
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