Citation: Yin Ling, Wang Anna, Zhao Meng, Sha Jingquan, Bai Yongxiang, Shi Haibin. Preparation of A Novel Smart Gold Nanorod Probe and Its Application in Cancer Cell Imaging[J]. Chemistry, ;2019, 82(1): 44-50. shu

Preparation of A Novel Smart Gold Nanorod Probe and Its Application in Cancer Cell Imaging

Yin Ling ^2,3 ,
Wang Anna ¹ ,
Zhao Meng ¹ ,
Sha Jingquan ³ ,
Bai Yongxiang ⁴ ,
Shi Haibin ^1,,

1.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123
2.
Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123
3.
Department of Chemistry and Chemical Engineering, Jining University, Qufu 273155
4.
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123

Corresponding author: Shi Haibin, bi@uda.edu.cn
Received Date: 8 August 2018
Accepted Date: 16 October 2018

Figures(5)

Developing a smart fluorescent probe which can specifically and sensitively detect matrix metalloproteinases-2 (MMP-2) is vital for the early accurate diagnosis and treatment of cancers. In this study, fluorescein isothiocyanate (FITC) was firstly conjugated to the peptide substrate GPLGVRGY by using copper (I)-catalyzed "click" reaction, and subsequently reacted with gold nanorods decorated with SH-PEG-COOH under the catalysis of EDC and Sulfo-NHS through amidation reaction to afford a novel fluorescence resonance energy transfer (FRET)-based probe GNR@FITC for specific detection of MMP-2. In vitro enzyme detection experiments, MTT assays and fluorescence imaging results showed that the probe shows good specificity and high sensitivity for the detection of MMP-2-overexpressed 4T1 cells, which may have great potential for clinical application in early diagnosis of breast cancer.
- Matrix metalloprotease-2(MMP-2),
- Gold nanorods,
- Fluorescence resonance energy transfer(FRET),
- Molecular probe,
- Fluorescent imaging
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