Citation: Qian DONG, Xiao Lei WANG, Lan Lan ZHU, Wen Rui JIN. Analysis of Amino Acids in a Single Human Red Blood Cell by Capillary Zone Electrophoresis with Intracellular NDA derivatization and Electrochemical Detection[J]. Chinese Chemical Letters, ;2002, 13(7): 655-657. shu

Analysis of Amino Acids in a Single Human Red Blood Cell by Capillary Zone Electrophoresis with Intracellular NDA derivatization and Electrochemical Detection

School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100

Received Date: 11 October 2001

A novel method for detcrmination of amino acids in individual human red blood cells has been dcveloped. In this method, the derivatization reagents (NDA and CN^-) are introduced into living cells by clcctroporation. After completion of derivatization, the amino acids in a single cell is determined by capillary zone electrophoresis with end-column ampcrometric detection.
- Capillary electrophorcsis,
- electrochemical detection,
- single cell analysis,
- amino acid
1. [1]
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2. [2]
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3. [3]
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4. [4]
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5. [5]
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7. [7]
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9. [9]
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12. [12]
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13. [13]
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14. [14]
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15. [15]
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17. [17]
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18. [18]
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19. [19]
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20. [20]
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