Citation: Yun Hui YANG, Ming Hui YANG, Jian Hui JIANG, Guo Li SHEN, Ru Qin YU. A Novel Biomolecular Immobilization Matrix Based on Nanoporous ZnO/Chitosan Composite Film for Amperometric Hydrogen Peroxide Biosensor[J]. Chinese Chemical Letters, ;2005, 16(7): 951-954. shu

A Novel Biomolecular Immobilization Matrix Based on Nanoporous ZnO/Chitosan Composite Film for Amperometric Hydrogen Peroxide Biosensor

1.
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
2.
College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, 650092

Received Date: 16 August 2004

Fund Project: This work was supported by the National Natural Science Foundation of China (Grants No. 20075006, 29975006, 20375012), the Foundation for Ph.D. Thesis Research (No. 20010532008), and the Foundation of Science Commission of Yunnan Province (No. 2002B0033M).

A novel ZnO/Chitosan composite matrix was developed to fabricate the H₂O₂ biosensor.This material combined the advantages of inorganic species, ZnO, and organic polymer, chitosan.Horseradish peroxidase immobilized in the material maintained its activity well as the usage of glutaraldehyde was avoided. The activity of enzyme was 7.9 times greater than the cross-linked enzyme. The parameters affecting the fabrication and experimental conditions of biosensors were optimized. With the aid of hydroquinone mediator, the biosensor had a fast response of less than 10s. The linear range was 5.0×10^-6 to 2.0×10^-3 mol/L with a sensitivity of 43.8 μAL/mmol.This matrix can also be used to immobilize other biomolecule.
- Nano ZnO,
- chitosan,
- composite film,
- biosensors,
- horseradish peroxidase
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