Citation: Jian Qiu MI, Xiao Hua QI, Xin Xiang ZHANG, Wen Bao CHANG. Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection[J]. Chinese Chemical Letters, ;2004, 15(8): 943-946. shu

Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection

The Key Lab of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871

Received Date: 21 July 2003

Fund Project: This work was financially supported by the National Natural Science Foundation of China (NNSFC) (20075001,20075003) and the Excellent Young Teachers Program of Ministry of Education, China. Some organic synthesis was completed in the lab of Prof. Jian-Bo Wang and thanks were also given here.

N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals.With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers.The detection limit was calculated to be 40 ng/mL.Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers.
- Polyclonal antibody,
- morphine,
- capillary electrophoresis immunoassay (CEIA),
- laser-induced fluorescence (LIF),
- specific
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