Citation: Shen Yang TONG, Na LI. A NEW STAINING METHOD FOR THE MEASUREMENT OF PROTEIN USING TETRAPHENYLPORPHYRIN TETRASULFONATE(TPPS₄)[J]. Chinese Chemical Letters, ;1993, 4(12): 1079-1080. shu

A NEW STAINING METHOD FOR THE MEASUREMENT OF PROTEIN USING TETRAPHENYLPORPHYRIN TETRASULFONATE(TPPS₄)

Shen Yang TONG ,
Na LI

Chemistry Department, Peking University, Beijing 100871

Received Date: 17 August 1993

Fund Project: This research was supported by grants from the National Natural Science foundation of China.

A new dye-staining method for protein assay is described. The reaction between TPPS₄ and protein molecule causes a shift in the absorption of TPPS₄ from 435 nm to 488 nm. The absorbance at 488 nm is proportional to the concentration of protein. The range of Beer's law was 1-5 ug/ml and the Sandell's sensitivity was 0.0087 ug/cm².
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