Citation: Pin YANG, Tian Xin WEI, Chun Ying, Jin Xi WANG, Da Pin FAN. A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator[J]. Chinese Chemical Letters, ;1996, 7(9): 820-822. shu

A New Fluorescence Method for the Measurement of Intracellular Free Rare Earth Ions:Fura-2 as an Indicator

a Institute of Molecular Science, ShanXi University, Taiyuan 030006;
b Chemical and Chemical Industry College of Nanjing University, Nanjing 210093;
c Medical College of NanJing University, NanJing 210093

Received Date: 29 February 1996

Fund Project: Supported by the National Natural Science Foundation of China.

Fura-2 was first used to monitor intracellular RE³⁺. The characterizations of RE³⁺-Fura-2 interactions in solutions simulating intracellular ionic composition show that La³⁺ and Y³⁺ both form 1:1 RE³⁺-fura-2 complexes. The apparent dissociation constants are 1.61×10^-7M, and 4.04×10^-7M, respectively. The mouse myeloma (SP2/0) cells exposed to free RE³⁺ or free RE³⁺ and some small molecules in body were studied by single cell cation measurement system. The results support the assumption that RE³⁺ ions cannot enter the cytoplasm of living cells, and the small molecules used in our experiments couldn't improve the permeability of RE³⁺ ions.
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