Citation: Qian WANG, Xu XU. DNA Separation by Capillary Electrophoresis with Ultraviolet Detection using Mixed Synthetic Polymers[J]. Chinese Chemical Letters, ;2003, 14(12): 1278-1280. shu

DNA Separation by Capillary Electrophoresis with Ultraviolet Detection using Mixed Synthetic Polymers

Qian WANG ,
Xu XU

State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032

Received Date: 2 December 2002

Fund Project: The Project is financially supported by the Innovation Fund (KSCX1-06) of Chinese Academy of Sciences.

The mixtures of two polymers, poly (N,N-dimethylacrylamide) (PDMA) and polyvinylpyrrolidone (PVP) were synthesized and used as the separation medium for double-stranded and single-stranded DNA fragments by capillary electrophoresis with UV detector. On optimal conditions, 2%w/v PDMA (2%w/v PVP can be used to separate the doublet 123/124bp in pBR322/Hae III Markers.
- Synthetic polymer,
- DNA separation,
- capillary electrophoresis,
- ultraviolet detection
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