Citation: Liang Quan SHENG, Shao Min LIU, Hou Rong XIAO, Bing Le XIA, Qing Liang LIU. A Novel Isoenzyme of CuZn-superoxide Dismutase from Nicotiana tobacum[J]. Chinese Chemical Letters, ;2004, 15(5): 543-546. shu

A Novel Isoenzyme of CuZn-superoxide Dismutase from Nicotiana tobacum

1.
Department of Chemistry, University of Science and Technology of China, Hefei 230026;
2.
Department of Food, University of Science and Technology of China, Hefei 230052

Received Date: 8 May 2003

An isoenzyme of CuZn-superoxide dismutase, denoted as CuZnSODⅢ, has been separated and purified from Nicotiana Tobacum (tobacco) leaves to apparent homogeneity. Its molecular mass is 22976.6Da. It is composed of one subunit, which is consisted of 187 amine acid residues and contains 1 copper and 0.5 zinc atom. The activation energy of the thermal denaturation process has been obtained as about 143.5kJmol^-1. Meanwhile, some properties of spectra were investigated.
- Superoxide dismutase,
- tobacco,
- purification,
- molecular properties
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