Citation: Juan Fang WU, Ling Xin CHEN, Guo An LUO, Yong Suo LIU, Yi Ming WANG, Zhi Hong JIANG. Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules[J]. Chinese Chemical Letters, ;2006, 17(3): 383-386. shu

Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

1.
Department of Chemistry, Tsinghua University, Beijing 100084;
2.
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong

Received Date: 22 August 2005

Fund Project: This work was supported by NNSF of China (No. 20475031), National "863" Project (No. 2002AA2Z2004) and Postdoctoral Science Foundation of China (No. 20040350303).

Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.
- Capillary zone electrophoresis,
- interaction,
- berberine,
- Hoechst 33258,
- DNA
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