Citation: Gu Ping TANG, Zhi Yu WANG. Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro[J]. Chinese Chemical Letters, ;2006, 17(1): 113-116. shu

Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro

Gu Ping TANG ^1,2, ,
Zhi Yu WANG ³

1.
College of Life-Science. ZheJiang University, Hangzhou 310028;
2.
Institute of Materials Research & Engineering, Singapore, 3 Research Link 117602;
3.
The Second Hospital of Zhejiang University, Hangzhou 310006

Received Date: 24 April 2005

Fund Project: This work was supported by the grants from the National High Biotechnology Development Program of China (2003AA216041), and the National Key Basic Research Program of China (2004CB518802).

The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa.Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5(mol/mol). The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.
- PEI-g-Comb-PEG,
- transferrin,
- gene therapy,
- transfection efficiency
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