Citation: Shui Sheng HONG, Jia CHEN, Jian Guo ZHANG, Yan Chun TAO, Lan Ying LIU. Purification and Structure Identification of Hyaluronic Acid[J]. Chinese Chemical Letters, ;2004, 15(7): 811-812. shu

Purification and Structure Identification of Hyaluronic Acid

1.
College of Life Science, Jilin University, Changchun 130023;
2.
State Key Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, ChangchunInstitute of Applied Chemistry, Changchun 130022;
3.
Key Laboratory of Supermolecular Structure and Spectroscopy of Educational Ministry, Jilin University, Changchun 130023

Received Date: 30 March 2003

Polysaccharide produced by mutated strain of Streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis, ¹³C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×10⁶D, which was measured by viscosity method.
- Streptococcus zooepidemicus,
- hyaluronic acid,
- structure identification
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