Citation: Qiang HE, Jun Bai LI. “Lotus” Domain Formation by the Hydrolysis Reaction of Phospholipase D to Phospholipid Monolayer[J]. Chinese Chemical Letters, ;2003, 14(11): 1199-1202.
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Hydrolysis reaction of L-α-dipalmitoylphosphatidylcholine (L-DPPC) monolayer with phospholipase D (PLD) has been investigated by Brewster angle microscopy (BAM) combined with the film balance. It has been found that the L-DPPC domains were changed into the "lotus" structure by PLD. It suggests that the hydrolysis reaction is incomplete and the products together with the nonreacted materials undergo a molecular rearrangement at the interface.
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