Citation: Jin Mao YOU, Xin Jun FAN, Qing Yu OU. HPLC of Amino Acids and Oligopeptides by Pre-Column Fluorescence Derivatization with 9-Acridine Formyl Chloride[J]. Chinese Chemical Letters, ;1997, 8(10): 875-878. shu

HPLC of Amino Acids and Oligopeptides by Pre-Column Fluorescence Derivatization with 9-Acridine Formyl Chloride

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences 730000

Received Date: 7 July 1997

A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by.pre-column fluorescence derivatization has been developed.Glycine.glycylglycine,histidine,triglycine and glutathione were separated on a reversed-phase C₁₈ column with methanol-water-triethylamine eluent,derivatization and chromatographic conditions were optimized.The five derivatives were eluted in 28 min with a good reproducibility.Linear range of the calibration graph was 0.08-260 nmol/ml^-1.The relative standard deviations(n=6) are < 5%.Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.
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