Citation: Tian Ying GUO, Yong Qing XIA, Guang Jie HAO, Bang Hua ZHANG. Chemically Modified Chitosan Beads as Molecularly Imprinted Polymer Matrix for Adsorptive Separation of Proteins[J]. Chinese Chemical Letters, ;2004, 15(11): 1339-1341. shu

Chemically Modified Chitosan Beads as Molecularly Imprinted Polymer Matrix for Adsorptive Separation of Proteins

State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071

Received Date: 19 September 2002

Fund Project: The authors were grateful to National Natural Science Foundation for financial support (Pro. No. 50003006).

In a phosphate buffer, a hemoglobin (Hb)-imprinted polymer complex was prepared using maleic anhydride (MAH) modified chitosan beads as matrix, acrylamide (AM) as functional monomer, N,N-methylenebisacrylamide (MBA) as cross-linker and potassiumpersulfate (KPS)/sodium hydrogen sulfite (NaHSO₃) as initiators. Langmuir analysis showed that an equal class of adsorption was formed in the molecular imprinting polymer (MIP), and the MIP has high adsorption capacity and selectivity for the imprinted molecule. The MIP can be reused and the recovery was approximately 100% at low concentration.
- Chemically modified chitosan beads,
- polyacrylamide,
- protein imprinting
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