Citation: Nong Yue HE, Chun YANG, Zheng Chun LIU, Zu Hong LU. Polyurethane Molecular Stamps for the in situ Synthesis of DNA Microarray[J]. Chinese Chemical Letters, ;2002, 13(9): 883-886. shu

Polyurethane Molecular Stamps for the in situ Synthesis of DNA Microarray

Nong Yue HE ^1,2,3, ,
Chun YANG ^1,2,3 ,
Zheng Chun LIU ^1,3 ,
Zu Hong LU ^1,3

1.
Key Laboratory for Molecular and Bio-molecular Electronics of Ministry of Education, Southeast University, Nanjing 210096;
2.
Key Lab of New Materials and Technology of China National Packaging Corporation, Zhuzhou Engineering College, Zhuzhou 412008;
3.
Department of Chemistry, Nanjing Normal University, Nanjing 210024

Received Date: 19 November 2001

Fabrication of polyurethane molecular stamps (PU stamps) based on polypropylene glycol (PPG) and toluene diisocyanate (TDI), using 3,3'-dichloro-4,4'-methylenedianiline (MOCA) as the crosslinker, is reported. It was shown from the contact angle measurement that PU stamps surface has good affinity with acetonitrile, guaranteeing the well distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays after hybridization confirmed polyurethane is an excellent material for molecular stamps when transferring polar chemicals and conducting reactions on interfaces by stamping.
- Molecular stamps,
- polyurethane,
- contact angle,
- soft lithography,
- DNA microarray
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