Citation: Xiao Rong RAN, Sheng Zhu SI, Qiong Lin LIANG, Guo An LUO. Application of the Reversed-phase Liquid Chromatography Coupled to Ion Trap Mass Spectrometry with Principal Components Analysis for Metabonomics Studies of Scutellarin in Rat Urine[J]. Chinese Chemical Letters, ;2006, 17(9): 1225-1228. shu

Application of the Reversed-phase Liquid Chromatography Coupled to Ion Trap Mass Spectrometry with Principal Components Analysis for Metabonomics Studies of Scutellarin in Rat Urine

1.
Department of Chemistry, Tsinghua University, Beijing 100084;
2.
Anhui Institution of Education, Hefei 230061

Received Date: 24 March 2006

Fund Project: This work was financially supported by the Chinese National Key Technologies R&D program (No. 2004BA721AB).

Metabonomics, a novel systemic approach, was applied to studies of Traditional Chinese Medicine scutellarin in rat urine. The liquid chromatography coupled with ion trap mass spectrometry combined with PCA was used in this paper. With this methodology, two potential metabolites of scutellarin were detected and the nine ions responsible for the gender variation and one ion for the dosage variation were found.
- Metabonomics,
- LC/MS,
- scutellarin,
- metabolites,
- endogenous
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