Citation: Hai Yan WANG, Dan Dan WANG, Chun Gui ZHAO, Ye Hong ZHOU, Shao Min SHUANG, Chuan DONG. Study on the Interferation of the Concentration of Free Calcium in Peripheral Human Lymphocyte by Low Dose Penicillin, Using Fura-2 as Fluorescent Probe[J]. Chinese Chemical Letters, ;2006, 17(5): 665-666. shu

Study on the Interferation of the Concentration of Free Calcium in Peripheral Human Lymphocyte by Low Dose Penicillin, Using Fura-2 as Fluorescent Probe

1.
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006;
2.
Medical College of Datong University, Datong 037000

Received Date: 8 October 2005

Fund Project: This work was supported by the National Natural Science Foundation of China (No.20575038) and the Foundation of Shanxi Provience (No.20031019).

The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2. The change of the free calcium concentration in cytosol was examined under different conditions. A characterization of Fura-2-Ca interaction in an isotonic saline solution showed that Ca2⁺ formed a 1:1 Fura-2-Ca complex with the apparent dissociation constant 1.81×10^-7 mol/L. The mechanism, by which penicillin induced the decrease of [Ca²⁺]_i, was discussed in detail. The low dose of penicillin might modify the lymphocytes' immunology response by interfering the increase in the intracellular free calcium concentration.
- Penicillin,
- lymphocytes,
- Fura-2,
- Ca²⁺,
- ouabain
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