Citation: Wei Guo HE, De Ying CHU, Jing Yue YANG, Dan Feng YAO, Mei Cheng SHAO. Lead (Ⅱ) Binding to Thionein[J]. Chinese Chemical Letters, ;1999, 10(1): 87-90. shu

Lead (Ⅱ) Binding to Thionein

College of Chemistry and Molecular Engineering Peking University, Beijing 100871

Received Date: 1 July 1998

Lead (Ⅱ)-binding properties of rabbit liver thionein 2 (apo-MT2) have been studied. Two kinds of complexes, Pb₇-MT and Pb₇-MT', are observed upon the binding of Pb²⁺ to apo-MT at different pH. From their characteristic peaks in UV and CD spectra, it is deduced that Pb²⁺ in Pb₇-MT is coordinated by four sulfhydryl groups in Td microsymmetry, while multiple coordination types including two-coordination maybe present in Pb₇-MT'.
- Thionein,
- lead (Ⅱ),
- UV spectra,
- circular dichroism,
- multiple coordination
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