Citation: Tong LIU, Xin Du GENG. The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography[J]. Chinese Chemical Letters, ;1999, 10(3): 219-222. shu

The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography

Tong LIU ,
Xin Du GENG

Institute of Modem Separation Science, Shaanxi Provincial Key Laboratory of Modern Separation Science, Northwest University, Xi'an 710069

Received Date: 26 October 1998

Fund Project: This work was supported by grants from National Natural Science Foundation of China

The separation efficiency of biopolymers with a short column in liquid chromatography has been investigated in this paper.It was found that the column length has slight effect on the resolution of biopolymers under gradient elution.The reasons have been explained by stoichiometric displacement model for retention of solute.The column 1 .0 cm long was also used in the separation and purification of recombinant human granulocyte colony-simulating factor (rhG-CSF).It only took 40 min and the purity by one step was found to be almost 100%.
- Short column,
- biopolymer,
- stoichiometric displacement model for retention,
- Z value
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