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Citation:
Bo YU, Jian HUANG, Xiao Feng CAI, Xiao Yong LIU, Cui Qing MA, Fu Li LI, Ping XU. A Novel Method for Conversion of Valuable BDS Intermediates HPBS/Cx-HPBS from DBT/Cx-DBT[J]. Chinese Chemical Letters,
;2005, 16(7): 935-938.
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A simple recombinant PCR method was used to delete the dszB gene responsible for the slowest step of the Dsz pathway and allow the accumulation of hydroxyphenyl benzene sulfinate (HPBS) in the recombinant E. coli. Using GC/MS, HPBS accumulation was confirmed. The recombinant E. coli could also desulfurize Cx-DBT to corresponding Cx-HPBS. The result gave a new insight to BDS process and explored a new method to obtain valuable surfactants from cheap raw materials.
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Keywords:
- Biodesulfurization,
- biosurfactant,
- recombinant PCR
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