Citation: Qing Bing ZENG, Qiao Zhen YE, Jia Rui XU, Ruo Wen FU. Ni²⁺-PAA Adsorbent for Purifying 6His-OmpTS Recombinant Protein[J]. Chinese Chemical Letters, ;2002, 13(5): 436-439. shu

Ni²⁺-PAA Adsorbent for Purifying 6His-OmpTS Recombinant Protein

1.
Department of chemistry, The First Military Medical University, Guangzhou 510275;
2.
College of Life science, Zhongshan University, Guangzhou 510275;
3.
Institute of Material Science, Zhongshan University, Guangzhou 510275

Received Date: 12 November 2000
Revised Date: 21 January 2002

Fund Project: The authors gratefully acknowledge financial support from the National Natural Scicncc Foundation of China, National Scicncc Fund lor Distinguished Young Scholars (grant Number: 59625307).

Functional Ni²⁺-polyacrylic acid (Ni²⁺-PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His-OmpTS that could be purified by Ni²⁺-PAA affinity chromatography.
- Affinity matrix,
- polyacrylic acid,
- chelate,
- nickel ion,
- hexa-histidine tagged
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