Citation: Qin WEI, Dan WU, Yan LI, Jun Hong WANG, Yan LIU, Bin DU. Microdetermination of Residual Protein in Penicillin by Resonance Light Scattering Technique with m-Nitrophenylfluorone-Mo(Ⅵ) Complex[J]. Chinese Chemical Letters, ;2005, 16(6): 815-818. shu

Microdetermination of Residual Protein in Penicillin by Resonance Light Scattering Technique with m-Nitrophenylfluorone-Mo(Ⅵ) Complex

Qin WEI ^1, ,
Dan WU ^1,2 ,
Yan LI ¹ ,
Jun Hong WANG ¹ ,
Yan LIU ¹ ,
Bin DU ¹

1.
School of Chemistry & Chemical Engineering, Jinan University, Jinan 250022;
2.
Key Laboratory of Colloid and Interface Chemistry, Shandong University, Jinan 250100

Received Date: 5 July 2004

Fund Project: Financial supports from the Natural Science Foundation of Shandong Province (Y2000B09) and Shandong Provincial Science and Technology Program (03C05) are gratefully acknowledged.

Based on the enhancement of resonance light scattering (RLS) of m-nitrophenylfluorone-Mo(Ⅵ) complex by protein, a novel method for protein microdetermination in Tween 20 microemulsion has been developed. Under optimum condition, the linear ranges of bovine serum albumin are 0~0.03 μg·mL^-1 with detection limits of 3.10 ng·mL^-1. Most of amino acids and metal ions do not interfere. The method can be applied to determination of residual protein in penicillin Na salt and penicillin G potassium salt samples with satisfactory results.
- Protein,
- microemulsion,
- m-NPF-Mo(VI)complex,
- penicillin,
- resonance light scattering
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