Citation: Yan WANG, Bo Lin GONG, Xin Du GENG. Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography[J]. Chinese Chemical Letters, ;2003, 14(8): 828-831. shu

Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography

Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shanxi Province, Northwest University, Xi'an 710069

Received Date: 5 September 2002

Fund Project: This work is supported by the National Natural Science Foundation (No.20175016).

Oxidative refolding of the denatured/reduced lysozyme was investigated by using weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent, sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL.
- Weak-cation exchange chromatography,
- lysozyme,
- protein refolding
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