Citation: Qiao-Ling Liu, Meng Fan, Wei Bian, Shao-Min Shuang, Chuan Dong. Synthesis of a new ratiometric emission Ca2+ indicator for in vivo bioimaging[J]. Chinese Chemical Letters, ;2013, 24(2): 156-158.
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A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized. The indicator exhibits a highly selective ratiometric emission response to Ca2+ over other metal cations and a large Stokes shift of 202 nm. Moreover, its practical cell imaging capability for intracellular Ca2+ in the resting-and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.
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Keywords:
- Ca2+ indicator,
- Ratiometric emission,
- Bioimaging
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