Citation: Qiao-Ling Liu, Meng Fan, Wei Bian, Shao-Min Shuang, Chuan Dong. Synthesis of a new ratiometric emission Ca²⁺ indicator for in vivo bioimaging[J]. Chinese Chemical Letters, ;2013, 24(2): 156-158. shu

Synthesis of a new ratiometric emission Ca²⁺ indicator for in vivo bioimaging

1.
a Research Center of Environmental Science and Engineering, Shanxi University, Taiyuan 030006, China;
2.
b Department of Chemistry, Taiyuan Normal University, Taiyuan 030031, China

Corresponding author: Chuan Dong,
Received Date: 4 November 2012
Available Online: 5 December 2012
Fund Project:

A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized. The indicator exhibits a highly selective ratiometric emission response to Ca²⁺ over other metal cations and a large Stokes shift of 202 nm. Moreover, its practical cell imaging capability for intracellular Ca²⁺ in the resting-and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.
- Ca²⁺ indicator,
- Ratiometric emission,
- Bioimaging
1. [1]
  [1] (a) M.J. Berridge, M.D. Bottman, P. Lipp, Calcium-a life and death signal, Nature 395 (1998) 645-648;
2. [2]
  (b) M. Nedergaard, A. Verkhratsky, Calcium dyshomeostasis and pathological calcium signalling in neurological diseases, Cell Calcium 47 (2010) 101-102;
3. [3]
  (c) N. Steinckwich, V. Schenten, C. Melchior, et al., An essential role of STIM1, Orai1, and S100A8-A9 proteins for Ca²⁺ signaling and FcγR-mediated phagosomal oxidative activity, J. Immunol. 186 (2011) 2182-2191.
4. [4]
  [2] (a) R.D. Burgoyne, Neuronal calcium sensor proteins: generating diversity in neuronal Ca²⁺ signaling, Nat. Rev. Neurosci. 8 (2007) 182-193;
5. [5]
  (b) P. Vito, E. Lacana, L. D0Aamio, Interfering with apoptosis: Ca²⁺-binding protein ALG-2 and Alzheimer's disease gene ALG-3, Science 271 (1996) 521-525.
6. [6]
  [3] (a) K. Satoh, T. Matsu-Ura, M. Enomoto, et al., Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells, J. Biol. Chem. 286 (2011) 20591-20599;
7. [7]
  (b) K.J. Buckler, Effects of exogenous hydrogen sulphide on calcium signalling, background (TASK) K channel activity and mitochondrial function in chemoreceptor cells, Pflugers Arch. 463 (2012) 743-754;
8. [8]
  (c) T.P. Collins, D.A. Terrar, Ca²⁺-stimulated adenylyl cyclases regulate the L-type Ca²⁺current in guinea-pig atrial myocytes, J. Physiol. 590 (2012) 1881-1893.
9. [9]
  [4] R.Y. Tsien, New calcium indicators and buffers with high selectivity against magnesium and protons: δesign, synthesis, and properties of prototype structures, Biochemistry 19 (1980) 2396-2404.
10. [10]
  [5] X.Z. Guo, X.M. Wang, Y.K. Du, et al., Synthesis and nonlinear optical properties of new quadrupolar chromophores, Chin. Chem. Lett. 16 (2005) 597-600.
11. [11]
  [6] G. Grynkiewicz, M. Poenie, R.Y. Tsien, A new generation of Ca²⁺ indicators with greatly improved fluorescence properties, J. Biol. Chem. 260 (1985), 3440-2450.
12. [12]
  [7] (a) A.M.B. Reeves, E. Shigetomi, B.S. Khakh, Bulk loading of calcium indicator dyes to study astrocyte physiology: key limitations and improvements using morphological maps, J. Neurosci. 31 (2011) 9353-9358;
13. [13]
  (b) M. Kamiya, K. Johnsson, Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore, Anal. Chem. 82 (2010) 6472-6479;
14. [14]
  (c) M. Collot, C. Loukou, A.V. Yakovlev, et al., Calcium rubies: a family of redemitting functionalizable indicators suitable for two-photon Ca²⁺ imaging, J. Am. Chem. Soc. 134 (2012) 14923-14931.
1. [1]
  Chuanfeng Fan , Jian Gao , Yingkai Gao , Xintong Yang , Gaoning Li , Xiaochun Wang , Fei Li , Jin Zhou , Haifeng Yu , Yi Huang , Jin Chen , Yingying Shan , Li Chen . A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging. Chinese Chemical Letters, 2024, 35(10): 109838-. doi: 10.1016/j.cclet.2024.109838
2. [2]
  Bin Fang , Jiaqi Yang , Limin Wang , Haoqin Li , Jiaying Guo , Jiaxin Zhang , Qingyuan Guo , Bo Peng , Kedi Liu , Miaomiao Xi , Hua Bai , Li Fu , Lin Li . A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury. Chinese Chemical Letters, 2024, 35(6): 108913-. doi: 10.1016/j.cclet.2023.108913
3. [3]
  Zengchao Guo , Weiwei Liu , Tengfei Liu , Jinpeng Wang , Hui Jiang , Xiaohui Liu , Yossi Weizmann , Xuemei Wang . Engineered exosome hybrid copper nanoscale antibiotics facilitate simultaneous self-assembly imaging and elimination of intracellular multidrug-resistant superbugs. Chinese Chemical Letters, 2024, 35(7): 109060-. doi: 10.1016/j.cclet.2023.109060
4. [4]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
5. [5]
  Ningning Gao , Yue Zhang , Zhenhao Yang , Lijing Xu , Kongyin Zhao , Qingping Xin , Junkui Gao , Junjun Shi , Jin Zhong , Huiguo Wang . Ba²⁺/Ca²⁺ co-crosslinked alginate hydrogel filtration membrane with high strength, high flux and stability for dye/salt separation. Chinese Chemical Letters, 2024, 35(5): 108820-. doi: 10.1016/j.cclet.2023.108820
6. [6]
  Simin Wei , Yaqing Yang , Junjie Li , Jialin Wang , Jinlu Tang , Ningning Wang , Zhaohui Li . The Mn/Yb/Er triple-doped CeO₂ nanozyme with enhanced oxidase-like activity for highly sensitive ratiometric detection of nitrite. Chinese Chemical Letters, 2024, 35(6): 109114-. doi: 10.1016/j.cclet.2023.109114
7. [7]
  Jun-Jie Fang , Zheng Liu , Yun-Peng Xie , Xing Lu . Superatomic Ag₅₈ nanoclusters incorporating a [MS₄@Ag₁₂]²⁺ (M = Mo or W) kernel show aggregation-induced emission. Chinese Chemical Letters, 2024, 35(10): 109345-. doi: 10.1016/j.cclet.2023.109345
8. [8]
  Jing-Jing Zhang , Lujun Lou , Rui Lv , Jiahui Chen , Yinlong Li , Guangwei Wu , Lingchao Cai , Steven H. Liang , Zhen Chen . Recent advances in photochemistry for positron emission tomography imaging. Chinese Chemical Letters, 2024, 35(8): 109342-. doi: 10.1016/j.cclet.2023.109342
9. [9]
  Hui Peng , Xiao Wang , Weiguo Huang , Shuiyue Yu , Linghang Kong , Qilin Wei , Jialong Zhao , Bingsuo Zou . Efficient tunable visible and near-infrared emission in Sb³⁺/Sm³⁺-codoped Cs₂NaLuCl₆ for near-infrared light-emitting diode, triple-mode fluorescence anti-counterfeiting and information encryption. Chinese Chemical Letters, 2024, 35(11): 109462-. doi: 10.1016/j.cclet.2023.109462
10. [10]
  Kun Zhang , Ni Dan , Dan-Dan Ren , Ruo-Yu Zhang , Xiaoyan Lu , Ya-Pan Wu , Li-Lei Zhang , Hong-Ru Fu , Dong-Sheng Li . A small D-A molecule with highly heat-resisting room temperature phosphorescence for white emission and anti-counterfeiting. Chinese Journal of Structural Chemistry, 2024, 43(3): 100244-100244. doi: 10.1016/j.cjsc.2024.100244
11. [11]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
12. [12]
  Chaoqun Ma , Yuebo Wang , Ning Han , Rongzhen Zhang , Hui Liu , Xiaofeng Sun , Lingbao Xing . Carbon dot-based artificial light-harvesting systems with sequential energy transfer and white light emission for photocatalysis. Chinese Chemical Letters, 2024, 35(4): 108632-. doi: 10.1016/j.cclet.2023.108632
13. [13]
  Bohan Zhang , Bingzhe Wang , Guichuan Xing , Zikang Tang , Songnan Qu . Regulation of the multi-emission centers in carbon dots via a bottom-up synthesis approach. Chinese Chemical Letters, 2024, 35(9): 109358-. doi: 10.1016/j.cclet.2023.109358
14. [14]
  Xingwen Cheng , Haoran Ren , Jiangshan Luo . Boosting the self-trapped exciton emission in vacancy-ordered double perovskites via supramolecular assembly. Chinese Journal of Structural Chemistry, 2024, 43(6): 100306-100306. doi: 10.1016/j.cjsc.2024.100306
15. [15]
  Yi Liu , Peng Lei , Yang Feng , Shiwei Fu , Xiaoqing Liu , Siqi Zhang , Bin Tu , Chen Chen , Yifan Li , Lei Wang , Qing-Dao Zeng . Topologically engineering of π-conjugated macrocycles: Tunable emission and photochemical reaction toward multi-cyclic polymers. Chinese Chemical Letters, 2024, 35(10): 109571-. doi: 10.1016/j.cclet.2024.109571
16. [16]
  Chun-Yun Ding , Ru-Yuan Zhang , Yu-Wu Zhong , Jiannian Yao . Binary and heterostructured microplates of iridium and ruthenium complexes: Preparation, characterization, and thermo-responsive emission. Chinese Journal of Structural Chemistry, 2024, 43(10): 100393-100393. doi: 10.1016/j.cjsc.2023.100393
17. [17]
  Shuo Li , Qianfa Liu , Lijun Mao , Xin Zhang , Chunju Li , Da Ma . Benzothiadiazole-based water-soluble macrocycle: Synthesis, aggregation-induced emission and selective detection of spermine. Chinese Chemical Letters, 2024, 35(11): 109791-. doi: 10.1016/j.cclet.2024.109791
18. [18]
  Yang Li , Yihan Chen , Jiaxin Luo , Qihuan Li , Yiwu Quan , Yixiang Cheng . Enhanced circularly polarized luminescence emission promoted by achiral dichroic oligomers of F8BT in cholesteric liquid crystal. Chinese Chemical Letters, 2024, 35(11): 109864-. doi: 10.1016/j.cclet.2024.109864
19. [19]
  Zhiqing Ge , Zuxiong Pan , Shuo Yan , Baoying Zhang , Xiangyu Shen , Mozhen Wang , Xuewu Ge . Novel high-temperature thermochromic polydiacetylene material and its application as thermal indicator. Chinese Chemical Letters, 2024, 35(11): 109850-. doi: 10.1016/j.cclet.2024.109850
20. [20]
  Lei Wang , Jun-Jie Wu , Chang-Cun Yan , Wan-Ying Yang , Zong-Lu Che , Xin-Yu Xia , Xue-Dong Wang , Liang-Sheng Liao . Near-infrared organic lasers with ultra-broad emission bands by simultaneously harnessing four-level and six-level systems. Chinese Chemical Letters, 2024, 35(8): 109365-. doi: 10.1016/j.cclet.2023.109365

Get Citation

PDF

XML

Metrics

PDF Downloads(547)
Abstract views(3043)
HTML views(102)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Synthesis of a new ratiometric emission Ca2+ indicator for in vivo bioimaging

Metrics

通讯作者: 陈斌, bchen63@163.com

Synthesis of a new ratiometric emission Ca²⁺ indicator for in vivo bioimaging