Citation: Yu Zhang, Yan-Jun Zhang, Xiao-Dong Xia, Xiao-Qi Hou, Cheng-Ting Feng, Jian-Xiu Wang, Liu Deng. A quantitative colorimetric assay of H₂O₂ and glucose using silver nanoparticles induced by H₂O₂ and UV[J]. Chinese Chemical Letters, ;2013, 24(12): 1053-1058. shu

A quantitative colorimetric assay of H₂O₂ and glucose using silver nanoparticles induced by H₂O₂ and UV

1.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Corresponding author: Jian-Xiu Wang, Liu Deng,
Received Date: 7 April 2013
Available Online: 1 July 2013
Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21105126) (No. 21105126)

A simple spectrophotometric assay of H₂O₂ and glucose using Ag nanoparticles has been carried out. Relying on the synergistic effect of H₂O₂ reduction and ultraviolet (UV) irradiation, Ag nanoparticles with enhanced absorption signals were synthesized. H₂O₂ served as a reducing agent in the Ag nanoparticles formation in which Ag⁺ was reduced to Ag⁰ by O₂^- generated via the decomposition of H₂O₂ in alkaline media. On the other hand, photoreduction of Ag⁺ to Ag⁰ under UV irradiations also contributed to the nanoparticles formation. The synthesized nanoparticles were characterized by TEM, XPS, and XRD. The proposed method could determine H₂O₂ with concentrations ranging from 5.0×10^-7 to 6.0×10^-5 mol/ L. The detection limit was estimated to be 2.0×10^-7 mol/L. Since the conversion of glucose to gluconic acid catalyzed by glucose oxidase was companied with the formation of H₂O₂, the sensing protocol has been successfully utilized for the determination of glucose in human blood samples. The results were in good agreement with those determined by a local hospital. This colorimetric sensor thus holds great promises in clinical applications.
- Silver nanoparticles,
- Colorimetric assay,
- H₂O₂,
- Glucose
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

A quantitative colorimetric assay of H2O2 and glucose using silver nanoparticles induced by H2O2 and UV

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通讯作者: 陈斌, bchen63@163.com

A quantitative colorimetric assay of H₂O₂ and glucose using silver nanoparticles induced by H₂O₂ and UV